Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABS1118 Anti-Cdc4 (FBXW7) Antibody, clone 9D8.1

Detect Cdc4 (FBXW7) using this mouse monoclonal Anti-Cdc4 (FBXW7) Antibody, clone 9D8.1, Cat. No. MABS1118, validated for use in Western Blotting.

Anti-Cdc4 (FBXW7) Antibody, clone 9D8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: FBXW7, AGO, CDC4, FBW7, FBX30, SEL10 UniProt Number: Q969H0
MABS1118
100 µL  
Purchase on Sigma-Aldrich

Különleges ajánlatok

Áttekintés

Replacement Information

Különleges ajánlatok

100% Performance Guaranteed

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1118
DescriptionAnti-Cdc4 (FBXW7) Antibody, clone 9D8.1
Alternate Names
  • F-box/WD repeat-containing protein 7
  • Archipelago
  • F-box and WD-40 domain protein 7
  • F-box protein FBW7
  • F-box protein FBX30
  • F-box protein SEL-10
  • hAgo
  • hCdc4
  • Homolog of C elegans sel-10
Background InformationF-box/WD repeat-containing protein 7 (UniProt Q969H0; also known as Archipelago, F-box and WD-40 domain protein 7, F-box protein FBW7, F-box protein FBX30, F-box protein SEL-10, hAgo, hCdc4, Homolog of C elegans sel-10) is encoded by the FBXW7 (also known as AGO, CDC4, FBW7, FBX30, SEL10) gene (Gene ID 55294) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. The F-box/WD repeat-containing protein 7 functions as an adaptor component of the Skp1, Cullin, F-box (SCF) and mediates substrates recruitment to the Cullin-RING E3 ubiquitin ligase (CRL) complex. Cyclin E, c-Myc, c-Jun, Notch1, SREBP, mTOR, c-Myb, MCL1, NF B2, and GATA3 are all known substrates targeted by Fbxw7 for ubiquitin-proteasome system- (UPS-) mediated degradation.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Cdc4 (FBXW7) using this mouse monoclonal Anti-Cdc4 (FBXW7) Antibody, clone 9D8.1, Cat. No. MABS1118, validated for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cdc4 (FBXW7) in 10 µg of NIH/3T3, HeLa, HepG2, K562, LNCaP, U2OS nuclear extracts.
Biological Information
ImmunogenGST-tagged recombinant human Cdc4 (FBXW7) isoform 1 (Archipelago alpha, FBW7alpha) N-terminal fragment.
Clone9D8.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 9D8.1 epitope lies within the N-terminal region specific to human and murine Cdc4 (FBXW7) spliced isoform 1 (Archipelago alpha, FBW7alpha, 110K, common), and not conserved in human/mouse isoform 2 (Archipelago beta, FBW7beta, 69K) or 3 (Archipelago gamma, FBW7gamma, Hippocampal). Note: UniProt Q8VBV4 currently lists only murine spliced isoform 3 (NM_080428), but not isoform 1 (NM_001177774) or 2 (NM_001177773).
IsotypeIgG1
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Specific ActivityN-terminal region.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FBXW7
  • AGO
  • CDC4
  • FBW7
  • FBX30
  • SEL10
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~80 kDa observed. 79.66 kDa (human; UniProt Q969H0-1) and mouse; NP_001171244 & NM_001177774) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cdc4 (FBXW7) in 10 µg of HEK293 nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABS1118 04054839081354