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MABE1669 Anti-Cas9 Antibody, NT Antibody, clone 8C1-F10

Anti-Cas9, NT, clone 8C1-F10 , Cat. No. MABE1669, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cas9/Csn1 and has been tested in Immunocytochemistry and Western Blotting.

Anti-Cas9 Antibody, NT Antibody, clone 8C1-F10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: cas9, csn1 UniProt Number: Q99ZW2
MABE1669
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
AICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1669
DescriptionAnti-Cas9 Antibody, NT Antibody, clone 8C1-F10
Alternate Names
  • CRISPR-associated endonuclease Cas9/Csn1
  • SpyCas9
Background InformationCRISPR-associated endonuclease Cas9/Csn1 (UniProt Q99ZW2; also known as SpyCas9) is encoded by the Streptococcus pyogenes serotype M1 gene Cas9 (also known as csn1) gene (Gene ID 901176). Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA loci containing short repetitions of base sequences complementary to antecedent mobile elements and target invading nucleic acids. Each repetition is followed by short segments of spacer DNA from previous exposures to a virus. Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1's CRISPR immune system that confers resistance to foreign genetic elements such as plasmids and phages. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems, correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and Cas9. Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3'-5' exonucleolytically.
References
Product Information
FormatPurified
PresentationPurified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Cas9, NT, clone 8C1-F10 , Cat. No. MABE1669, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cas9/Csn1 and has been tested in Immunocytochemistry and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected exogenously expressed full-length nuclease-dead Cas9 in transfected NIH/3T3 cells fixed with 4% paraformaldehyde and permeabilized by 0.3% Triton X-100.
Biological Information
ImmunogenHis-tagged recombinant Cas9 N-terminal fragment excluding the active site RuvC-I.
Clone8C1-F10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 8C1-F10 targets an epitope within Cas9 N-terminal region outside the active site RuvC-I.
IsotypeIgG2bκ
Species Reactivity
  • All
Species Reactivity NoteStreptococcus pyogenes.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • cas9
  • csn1
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~160 kDa observed. 158.4 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH3T3 cells.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected exogenously expressed full-length nuclease-dead Cas9 in 10 µg of lysate from transfected NIH/3T3 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABE1669 04054839111723