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PC420 Anti-Capsaicin Receptor (Ab-1) (824-838) Rabbit pAb

Anti-Capsaicin Receptor (Ab-1) (824-838), rabbit polyclonal, recognizes the ~100 kDa capsaicin receptor in mouse spinal cord extract. It is validated for WB, IF & IHC in frozen and paraffin sections.

Anti-Capsaicin Receptor (Ab-1) (824-838) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Vanilloid Receptor

PC420
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityHostAntibody Type
M, RRbPolyclonal Antibody
Description
OverviewRecognizes the ~100 kDa capsaicin receptor protein in mouse spinal cord extract.
Catalogue NumberPC420
Brand Family Calbiochem®
SynonymsAnti-Vanilloid Receptor
Application Data
Detection of mouse capsaicin receptor by staining frozen sections. Sample: Mouse spinal cord dorsal horn. Primary antibody: Anti-Capsaicin Receptor (Ab-1) (824-838) Rabbit pAb (Cat. No. PC420) (5 μg/ml, panel B or 10 µg/ml, panel C) and normal rabbit IgG (5 µg/ml, panel A). Detection: DAB.

Detection of mouse capsaicin receptor by immunoblotting. Sample: Whole tissue extract 60 µg) from mouse spinal cord (lanes 1,3,4; MWM, lane 2). Primary antibody: Anti-Capsaicin Receptor (Ab-1) (824-838) Rabbit pAb (Cat. No. PC420) (5 µg/ml, lane 3 or 10 µg/ml, lane 4) and Normal rabbit IgG (5 µg/ml, lane 1). Detection: chemiluminescence.
References
ReferencesCaterina, M.J., et al. 1999. Nature 398, 436.
Gau, A., et al. 1999. Eur. J. Neurosci. 11, 946.
Michael, G.J., and Priestley, J.V. 1999. J. Neurosci. 19, 1844.
Caterina, M.J., et al. 1997. Nature 389, 816.
Szallasi, A. 1994. Gen. Pharmacol. 25, 223.
Beven, S., and Szolcsanyi, J. 1990. Trends Pharmacol. Sci. 11, 330.
Fields, H.L. 1987. Pain (New York: McGraw-Hill).
Product Information
FormLiquid
FormulationIn PBS.
Positive controlMouse spinal cord dorsal horn
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesFrozen Sections Schicho, R., et al. 2004. Eur. J. Neurosci. 19, 1811.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Paraffin Sections
Application NotesFrozen Sections (5 µg/ml, see application references)
Immunoblotting (5-10 µg/ml)
Immunofluorescence (2 µg/ml)
Paraffin Sections (5 µg/ml; no pre-treatment required)
Application CommentsImmunohistochemistry was performed on mouse spinal cord sections fixed in 4% paraformaldehyde. Immunohistochemical staining results correlate well with other independent reports and in situ hybridization studies. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (EDAEVFKDSMVPGEK) corresponding to amino acids 824-838 of rat capsaicin receptor, conjugated to KLH
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
PC420 0

Documentation

Anti-Capsaicin Receptor (Ab-1) (824-838) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Capsaicin Receptor (Ab-1) (824-838) Rabbit pAb Certificates of Analysis

TitleLot Number
PC420

References

Hivatkozások áttekintése
Caterina, M.J., et al. 1999. Nature 398, 436.
Gau, A., et al. 1999. Eur. J. Neurosci. 11, 946.
Michael, G.J., and Priestley, J.V. 1999. J. Neurosci. 19, 1844.
Caterina, M.J., et al. 1997. Nature 389, 816.
Szallasi, A. 1994. Gen. Pharmacol. 25, 223.
Beven, S., and Szolcsanyi, J. 1990. Trends Pharmacol. Sci. 11, 330.
Fields, H.L. 1987. Pain (New York: McGraw-Hill).

Citations

Titulus
  • S. Tamura, Y. Morikawa and E. Senba. (2005) TRPV2, a capsaicin receptor homologue, is expressed predominantly in the neurotrophin-3-dependent subpopulation of primary sensory neurons. Neuroscience 130, 223-228.
  • Yi Dai, et al. (2004) Proteinase-activated receptor 2-mediated potentiation of transient receptor potential vanilloid subfamily 1 activity reveals a mechanism for proteinase-induced inflammatory pain. Journal of Neuroscience 24, 4293-4299.
  • Rudolf Schicho, et al. (2004) Increased Expression of TRPV1 Receptor in Dorsal Root Ganglia by Acid Insult of the Rat Gastric Mucosa. European Journal of Neuroscience 19, 1811-1818.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision02-May-2008 JSW
    SynonymsAnti-Vanilloid Receptor
    ApplicationFrozen Sections (5 µg/ml, see application references)
    Immunoblotting (5-10 µg/ml)
    Immunofluorescence (2 µg/ml)
    Paraffin Sections (5 µg/ml; no pre-treatment required)
    Application Data
    Detection of mouse capsaicin receptor by staining frozen sections. Sample: Mouse spinal cord dorsal horn. Primary antibody: Anti-Capsaicin Receptor (Ab-1) (824-838) Rabbit pAb (Cat. No. PC420) (5 μg/ml, panel B or 10 µg/ml, panel C) and normal rabbit IgG (5 µg/ml, panel A). Detection: DAB.

    Detection of mouse capsaicin receptor by immunoblotting. Sample: Whole tissue extract 60 µg) from mouse spinal cord (lanes 1,3,4; MWM, lane 2). Primary antibody: Anti-Capsaicin Receptor (Ab-1) (824-838) Rabbit pAb (Cat. No. PC420) (5 µg/ml, lane 3 or 10 µg/ml, lane 4) and Normal rabbit IgG (5 µg/ml, lane 1). Detection: chemiluminescence.
    DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~100 kDa capsaicin receptor protein.
    BackgroundThe detection of noxious stimuli (chemical, mechanical, or thermal), a process referred to as nociception, occurs predominantly at the peripheral terminals of primary afferent neurons. This information is ultimately transmitted to the central nervous system to evoke a perception of pain which initiates appropriate protective reflexes. Vanilloid-containing compounds which include capsaicin, the active ingredient in hot chilli peppers, selectively depolarize nociceptors to activate the "nociceptive" pathway. The receptor for capsaicin, vanilloid receptor 1 (VR1), has been identified as a nonselective cation channel that resembles members of the transient receptor potential (TRP) family of ion channels. The vanilloid receptor 1 protein functions both as a receptor for capsaicin and a transducter of noxious thermal stimuli. Immunocytochemical and mRNA localization studies suggest that VR1 protein is localized to small-diameter sensory neurons within the dorsal root ganglia and nerve terminals in the dorsal horn. A capsaicin-receptor homolog has recently been identified, designated the vanilloid-receptor-like protein 1 (VRL-1). VRL-1 is activated by high temperatures and is expressed in medium- to large-diameter neurons within sensory ganglia.
    HostRabbit
    Immunogen speciesRat
    Immunogena synthetic peptide (EDAEVFKDSMVPGEK) corresponding to amino acids 824-838 of rat capsaicin receptor, conjugated to KLH
    IsotypeIgG
    Speciesmouse, rat
    Positive controlMouse spinal cord dorsal horn
    FormLiquid
    FormulationIn PBS.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsImmunohistochemistry was performed on mouse spinal cord sections fixed in 4% paraformaldehyde. Immunohistochemical staining results correlate well with other independent reports and in situ hybridization studies. Antibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesCaterina, M.J., et al. 1999. Nature 398, 436.
    Gau, A., et al. 1999. Eur. J. Neurosci. 11, 946.
    Michael, G.J., and Priestley, J.V. 1999. J. Neurosci. 19, 1844.
    Caterina, M.J., et al. 1997. Nature 389, 816.
    Szallasi, A. 1994. Gen. Pharmacol. 25, 223.
    Beven, S., and Szolcsanyi, J. 1990. Trends Pharmacol. Sci. 11, 330.
    Fields, H.L. 1987. Pain (New York: McGraw-Hill).
    Citation
  • S. Tamura, Y. Morikawa and E. Senba. (2005) TRPV2, a capsaicin receptor homologue, is expressed predominantly in the neurotrophin-3-dependent subpopulation of primary sensory neurons. Neuroscience 130, 223-228.
  • Yi Dai, et al. (2004) Proteinase-activated receptor 2-mediated potentiation of transient receptor potential vanilloid subfamily 1 activity reveals a mechanism for proteinase-induced inflammatory pain. Journal of Neuroscience 24, 4293-4299.
  • Rudolf Schicho, et al. (2004) Increased Expression of TRPV1 Receptor in Dorsal Root Ganglia by Acid Insult of the Rat Gastric Mucosa. European Journal of Neuroscience 19, 1811-1818.
    		•
    Application referencesFrozen Sections Schicho, R., et al. 2004. Eur. J. Neurosci. 19, 1811.