Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

208882 Anti-Calnexin, N-Terminal (50-68) Rabbit pAb

Anti-Calnexin, N-Terminal (50-68) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-IP90, Anti-p88

208882
  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~90 kDa calnexin protein.
Catalogue Number208882
Brand Family Calbiochem®
SynonymsAnti-IP90, Anti-p88
Application Data
Detection of human calnexin by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysate from Vero cells (lane 2), 3T3 cells (lane 3), PC-12 cells (lane 4), and HeLa cells (lane 5). Primary antibody: Anti-Calnexin, N-Terminal (50-68) Rabbit pAb (Cat. No. 208882) (1:1000). Detection: chemiluminescence.
References
ReferencesLe, A., et al. 1994. J. Biol. Chem. 269, 7514.
David, V., et al. 1993. J. Biol. Chem. 268, 9585.
Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000, colorimetric)
Application CommentsVariables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide (DTSAPTSPKVTYKAPVPSG) corresponding to amino acids 50-68 of canine calnexin, conjugated to KLH
ImmunogenCanine
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
208882 0

Documentation

Anti-Calnexin, N-Terminal (50-68) Rabbit pAb Certificates of Analysis

TitleLot Number
208882

References

Hivatkozások áttekintése
Le, A., et al. 1994. J. Biol. Chem. 269, 7514.
David, V., et al. 1993. J. Biol. Chem. 268, 9585.
Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-October-2009 JSW
SynonymsAnti-IP90, Anti-p88
ApplicationImmunoblotting (1:1000, colorimetric)
Application Data
Detection of human calnexin by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysate from Vero cells (lane 2), 3T3 cells (lane 3), PC-12 cells (lane 4), and HeLa cells (lane 5). Primary antibody: Anti-Calnexin, N-Terminal (50-68) Rabbit pAb (Cat. No. 208882) (1:1000). Detection: chemiluminescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~90 kDa calnexin protein.
BackgroundCalnexin is an ER transmembrane protein that transiently associates with newly synthesized, incompletely folded monomeric glycoproteins. Calnexin associates with the ER membrane phosphoglycoprotein, signal sequence receptor SSRµ, and has been identified as the ER protein associated with major histocompatibility complex (MHC) class I heavy chain and with the T-cell receptor and membrane immunoglobulin before assembly of these multimeric complexes. Hence, calnexin may represent a molecular chaperone in the ER membrane that is selective for incompletely folded oligomeric membrane proteins newly translocated to the ER.
HostRabbit
Immunogen speciesCanine
Immunogena synthetic peptide (DTSAPTSPKVTYKAPVPSG) corresponding to amino acids 50-68 of canine calnexin, conjugated to KLH
IsotypeIgG
Speciesbovine, canine, guinea pig, hamster, human, monkey, mouse, porcine, rabbit, rat, sheep
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Preservative≤0.1% sodium azide
CommentsVariables associated with assay conditions will dictate the optimal working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesLe, A., et al. 1994. J. Biol. Chem. 269, 7514.
David, V., et al. 1993. J. Biol. Chem. 268, 9585.
Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.