Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PC253L Anti-Calbindin D-28K (Ab-1) Rabbit pAb

Anti-Calbindin D-28K (Ab-1), rabbit polyclonal, reecognizes the Calbindin D-28K protein in rat hippocampus. It is validated for use in frozen sections and for T cell co-stimulation.

Anti-Calbindin D-28K (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC253L
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityHostAntibody Type
Bab, H, Mk, RRbPolyclonal Antibody

Products

KatalógusszámCsomagolás Menny./csomag
PC253L-100UL Muanyagampulla 100 ul
Description
OverviewRecognizes the Calbindin D-28K protein in rat hippocampus tissue.
Catalogue NumberPC253L
Brand Family Calbiochem®
References
ReferencesConde, F., et al. 1994. J. Comp. Neurol. 341, 95.
Heizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15.
Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat striatum, hippocampus, or cortex
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesFrozen and Vibrotome Sections Raven, M.A., et al. 2005. Vis. Neurosci. 22, 569.
Key Applications Frozen Sections
T Cell Co-stimulation
Application NotesFrozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)
Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)
Application CommentsAntibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified bovine cerebellum calbindin D-28K protein
ImmunogenBovine
HostRabbit
IsotypeIgG
Species Reactivity
  • Baboon
  • Human
  • Monkey
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
PC253L-100UL 04055977224702

Documentation

Anti-Calbindin D-28K (Ab-1) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Calbindin D-28K (Ab-1) Rabbit pAb Certificates of Analysis

TitleLot Number
PC253L

References

Hivatkozások áttekintése
Conde, F., et al. 1994. J. Comp. Neurol. 341, 95.
Heizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15.
Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.

Citations

Titulus
  • Mary. A. Raven, et al. (2005) Developmental Improvement in the Regularity and Packing of Mouse Horizontal Cells: Implications for Mechanisms Underlying Mosaic Pattern Formation. Visual Neuroscience 22, 569-573.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision09-October-2007 RFH
    ApplicationFrozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)
    Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)
    DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the calbindin D-28K protein.
    BackgroundSeveral EF-hand calcium-binding proteins have been identified within the nervous system, which includes calbindin, calretinin, and parvalbumin. These cytosolic calcium-binding proteins modulate and mediate the function of the intracellular second messenger calcium. These proteins are believed to function in the buffering, transport and regulation of calcium. Calbindin, calretinin, and parvalbumin are localized in distinct neuronal subpopulations, and antibodies against these proteins are useful markers for neuranatomical and developmental studies.
    HostRabbit
    Immunogen speciesBovine
    Immunogenpurified bovine cerebellum calbindin D-28K protein
    IsotypeIgG
    Speciesbaboon, human, monkey, rat
    Positive controlRat striatum, hippocampus, or cortex
    FormLyophilized
    FormulationUndiluted serum.
    Preservative≤0.1% sodium azide
    CommentsAntibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
    Toxicity Standard Handling
    ReferencesConde, F., et al. 1994. J. Comp. Neurol. 341, 95.
    Heizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15.
    Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
    Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.
    Citation
  • Mary. A. Raven, et al. (2005) Developmental Improvement in the Regularity and Packing of Mouse Horizontal Cells: Implications for Mechanisms Underlying Mosaic Pattern Formation. Visual Neuroscience 22, 569-573.
    		•
    Application referencesFrozen and Vibrotome Sections Raven, M.A., et al. 2005. Vis. Neurosci. 22, 569.