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681509 Anti-CaV1.3 (809-825) Rabbit pAb

Anti-CaV1.3 (809-825) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-L-Type Ca2+ Channel

681509
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes all forms of the α1D-subunit of Cav1.3 voltage-gated calcium channel. Supplied with a control peptide.
Note: The α-subunit is highly sensitive to proteases. Please refer to the product insert for proper sample preparation.
Catalogue Number681509
Brand Family Calbiochem®
SynonymsAnti-L-Type Ca2+ Channel
Application Data
Detection of rat CaV1.3 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV1.3 (809-825) Rabbit pAb (Cat. No. 681509) (1:200). Detection: chemilumiescence.

Detection of rat CaV1.3 by immunohistochemistry. Samples: Adult rat dorsal root ganglion with staining appearing in clusters of cells (green) but not in axons. Staining of neurofilament 200 demonstrates partial overlap with CaV1.3 in neuronal staining but not in axons (red). Primary antibody: Anti-CaV1.3 (809-825) Rabbit pAb (Cat. No. 681509) (1:100). Detection: fluorescence.
References
ReferencesHell, J.W., et al. 1993. J. Cell Biol. 123, 949.
Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
Hui, A., et al. 1991. Neuron 7, 35.
Product Information
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 5% sucrose, 1% BSA, 0.05% sodium azide, pH. 7.4 and 40 µg control peptide lyophilized from PBS.
Preservative≤0.1% sodium azide (antibody only)
Applications
Key Applications Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesImmunoblotting (1:200)
Immunohistochemistry (1:100)
Application CommentsThe epitope is specific for α1D-subunits of the voltage-gated Ca2+ channel and is not present in any other known protein. It is highly conserved in human and hamster protein but cross-reactivity has not been tested. Antibody binding can be fully inhibited with the soluble CP809-825 blocking peptide (supplied). For use as a negative control, preincubate 1 µg control peptide with 1 µg antibody for 1 hr at room temperature. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml). To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide [(KY)DNKVTIDDYQEEAEDKD] corresponding to amino acids 809-825 of the α1D-subunit of the rat brain voltage-gated calcium channels
ImmunogenRat
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the antibody with 50 µl dH₂O. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the peptide with 100 µl dH₂O, aliquot, and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
681509 0

Documentation

Anti-CaV1.3 (809-825) Rabbit pAb Certificates of Analysis

TitleLot Number
681509

References

Hivatkozások áttekintése
Hell, J.W., et al. 1993. J. Cell Biol. 123, 949.
Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
Hui, A., et al. 1991. Neuron 7, 35.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-November-2009 RFH
SynonymsAnti-L-Type Ca2+ Channel
ApplicationImmunoblotting (1:200)
Immunohistochemistry (1:100)
Application Data
Detection of rat CaV1.3 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV1.3 (809-825) Rabbit pAb (Cat. No. 681509) (1:200). Detection: chemilumiescence.

Detection of rat CaV1.3 by immunohistochemistry. Samples: Adult rat dorsal root ganglion with staining appearing in clusters of cells (green) but not in axons. Staining of neurofilament 200 demonstrates partial overlap with CaV1.3 in neuronal staining but not in axons (red). Primary antibody: Anti-CaV1.3 (809-825) Rabbit pAb (Cat. No. 681509) (1:100). Detection: fluorescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes all forms of the α1D-subunit of the voltage-gated Ca channel. Supplied with a control peptide.
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide [(KY)DNKVTIDDYQEEAEDKD] corresponding to amino acids 809-825 of the α1D-subunit of the rat brain voltage-gated calcium channels
IsotypeIgG
Speciesmouse, rat
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 5% sucrose, 1% BSA, 0.05% sodium azide, pH. 7.4 and 40 µg control peptide lyophilized from PBS.
Preservative≤0.1% sodium azide (antibody only)
CommentsThe epitope is specific for α1D-subunits of the voltage-gated Ca2+ channel and is not present in any other known protein. It is highly conserved in human and hamster protein but cross-reactivity has not been tested. Antibody binding can be fully inhibited with the soluble CP809-825 blocking peptide (supplied). For use as a negative control, preincubate 1 µg control peptide with 1 µg antibody for 1 hr at room temperature. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml). To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the antibody with 50 µl dH₂O. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the peptide with 100 µl dH₂O, aliquot, and freeze (-20°C).
Toxicity Standard Handling
ReferencesHell, J.W., et al. 1993. J. Cell Biol. 123, 949.
Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
Hui, A., et al. 1991. Neuron 7, 35.