Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABC1736-100UG Anti-CIDE-A Antibody, clone V62P1E3 B10

Anti-CIDE-A, clone V62P1E3 B10, Cat. No. MABC1736, is a mouse monoclonal antibody that detects CIDE-A and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

MABC1736-100UG
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABC1736-100UG
DescriptionAnti-CIDE-A Antibody, clone V62P1E3 B10
Alternate Names
  • Cell death activator CIDE-A
  • Cell death-inducing DFFA-like effector A
Background InformationCell death activator CIDE-A (UniProt: O60543; also known as Cell death-inducing DFFA-like effector A) is encoded by the CIDEA gene (Gene ID: 1149) in human. Three members of the CIDE family of proteins have been described in human CIDE-A, CIDE-B, and CIDE-C. They all contain a common N-terminal CIDE-N domain and a C-terminal CIDE-C domain. CIDE-A is a brown fat-associated protein that has been implicated in lipid and energy metabolism. Its expression has been reported in colon and normal breast tissue. It binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. It is shown to be enriched at lipid droplet contact sites and at focal contact sites between lipid droplets where it promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. It is reported to strongly promote triglyceride accumulation even in cell types that do not normally store large amounts of neutral lipid. Its expression levels in adipose tissue have been correlated with insulin sensitivity, which could be related to enhanced storage of triglyceride resulting in reduced levels of fatty acid levels in the circulation. CIDE-A is also reported to acts as a CCAAT Enhancer Binding Protein b (CEBPB) coactivator in mammary epithelial cells and controls the expression of CEBPB downstream target genes, such as ID2, IGF1, PRLR, SOCS1, SOCS3, XDH. Higher expression of CIDE-A has been reported in colorectal tumors compared to normal colonic mucosa. (Ref.: Alnabulsi, A., et al. (2019). Int. J. Cancer. 145(4);1138-1147; Puri, V., et al. (2008). Proc. Natl. Acad. Sci. USA. 105(22); 7833-7838).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-CIDE-A, clone V62P1E3 B10, Cat. No. MABC1736, is a mouse monoclonal antibody that detects CIDE-A and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • ELISA
  • Western Blotting
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected CIDE-A in Western Blotting applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

ELISA Analysis: A representative lot detected CIDEA in ELISA applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

Immunohistochemistry Applications: A representative lot detected CIDEA in Immunohistochemistry applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to 9 amino acids from the C-terminal region of human CIDE-A.
EpitopeC-terminal
CloneV62P1E3 B10
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone V62P1E3 B10 is a mouse monoclonal antibody that detects human Cell death activator CIDE-A. It targets an epitope within 9 amino acids from the C-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CIDEA
Purification MethodProtein G purified
UniProt Number
Molecular Weight24.69 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human colon cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected CIDE-A in human colon cancer tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABC1736-100UG 04065267060392