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MABF2079 Anti-CD8 alpha Antibody, clone 53-6.72

This rat monoclonal Anti-CD8 alpha, clone 53-6.72, Cat. No. MABF2079, is a rat monoclonal antibody that detects CD8 alpha and has been tested for use in Flow Cytometry and functional studies.

Anti-CD8 alpha Antibody, clone 53-6.72 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CD8a, Lyt2, Lyt-2 UniProt Number: P01731
MABF2079
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, Function AssayRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2079
DescriptionAnti-CD8 alpha Antibody, clone 53-6.72
Alternate Names
  • T-cell surface glycoprotein CD8 alpha chain
  • T-cell surface glycoprotein Lyt-2
Background InformationT-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as T-cell surface glycoprotein Lyt-2, CD8a) is encoded by the CD8a (also known as Lyt-2, Lyt2) gene (Gene ID: 12525) in murine species. CD8 alpha is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved to form the mature protein that has an extracellular domain (aa 28-196), a helical domain (aa 197-217), and a cytoplasmic tail (aa 218-247). CD8 is generally present as a heterodimer of alpha and beta chains linked by two disulfide bonds, however, it can also form homodimers. The CD8 alpha/alpha homodimer is expressed on a subset of gamma/delta TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 plays a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. CD8 acts as a co-receptor for the T cell receptor (TCR) and like the TCR, it binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in PBS without azide.
Quality LevelMQ100
Applications
ApplicationThis rat monoclonal Anti-CD8 alpha, clone 53-6.72, Cat. No. MABF2079, is a rat monoclonal antibody that detects CD8 alpha and has been tested for use in Flow Cytometry and functional studies.
Key Applications
  • Flow Cytometry
  • Function Assay
Application NotesFlow Cytometry Analysis: A representative lot detected CD8 alpha in Flow Cytometry applications (de Latour, R.P., et. al. (2010). Blood. 116(20):4175-84).

Functional Analysis: A representative lot detected CD8 alpha in Functional analysis applications (Daley, D., et. al. (2016). Cell. 166(6):1485-1499; Hannani, D., et. al. (2014). Cell Death Differ. 21(1):50-8).
Biological Information
ImmunogenMurine Thymic/splenic lymphocytes.
Clone53-6.72
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 53-6.72 is a rat monoclonal antibody that detects CD8 alpha chain in murine thymocytes.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD8a
  • Lyt2
  • Lyt-2
Purification MethodProtein G purified
UniProt Number
Molecular Weight27.46 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in mouse thymocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected CD8 alpha in one million mouse thymocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABF2079 04054839355622