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MABF2032 Anti-CD31 (PECAM-1) Antibody, clone MBC 78.1

Anti-CD31 (PECAM-1), clone MBC 78.1, Cat. No. MABF2032, is a mouse monoclonal antibody that detects CD31/PECAM-1 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Immunoprecipitation.

Anti-CD31 (PECAM-1) Antibody, clone MBC 78.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: PECAM1 UniProt Number: P16284
MABF2032
100 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2032
DescriptionAnti-CD31 (PECAM-1) Antibody, clone MBC 78.1
Alternate Names
  • Platelet endothelial cell adhesion molecule
  • PECAM-1
  • EndoCAM. GPIIA
  • PECA1
  • CD31
Background InformationPlatelet endothelial cell adhesion molecule (UniProt: P16284; also known as PECAM-1, EndoCAM. GPIIA', PECA1, CD31) is encoded by the PECAM1 gene (Gene ID: 5175) in human. PECAM-1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved to generate the mature protein with a large extracellular region (aa 28-601) that contains six Ig-like C2-type domains, followed by a transmembrane segment (aa 602-620) and a cytoplasmic tail (aa. 621-738 of isoform Long) that plays an essential role in PECAM-1-mediated cellular signaling. PECAM-1 is essential for leukocyte transendothelial migration (TEM) under most inflammatory conditions. It is expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Six isoforms of PECAM-1 have been described that are produced by alternative splicing. Isoform Long predominates in all tissues examined, whereas Delta 12 isoform is detected only in trachea and isoform Delta14-15 are only detected in lung tissue. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia and U-937 histiocytic lymphoma cell lines. Tyrosine 690 residue plays a critical role in TEM and is required for efficient trafficking of PECAM-1 to and from the lateral border recycling compartment (LBRC) and is also required for the LBRC membrane to be targeted around migrating leukocytes. PECAM-1 also prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CD31 (PECAM-1), clone MBC 78.1, Cat. No. MABF2032, is a mouse monoclonal antibody that detects CD31/PECAM-1 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Immunoprecipitation.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected CD31 (PECAM-1) in THP-1 cells.

Immunoprecipitation Analysis: A representative lot detected CD31 (PECAM-1) in Immunoprecipitation applications (DeLisser, H.M., et. al. (1994). J Cell Biol. 124(1-2):195-203).

Flow Cytometry Analysis: A representative lot detected CD31 (PECAM-1) in Flow Cytometry applications (DeLisser, H.M., et. al. (1994). J Cell Biol. 124(1-2):195-203).

Flow Cytometry Analysis: 1 µg from a representative lot detected CD31 (PECAM-1) in one millioin THP-1 cells.
Biological Information
ImmunogenPECAM-1 purified from human platelets.
CloneMBC 78.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone MBC 78.1 detects CD31/PECAM-1 in human cells. It targets an epitope within the IgG homology domain 5.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PECAM1
Purification MethodProtein G purified
UniProt Number
Molecular Weight82.54 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in Jurkat cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CD31 (PECAM-1) in Jurkat cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABF2032 04054839355585