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MABE664-25UG Anti-CBX1 (HP1 beta) Antibody, clone 5E9.1

Anti-CBX1 (HP1 beta), clone 5E9.1 Antibody, Cat. No. MABE664-25UG, is a highly specific mouse monoclonal antibody, that targets CBX1 and has been tested in Western Blotting.

Anti-CBX1 (HP1 beta) Antibody, clone 5E9.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: CBX1, CBX UniProt Number: P83916
MABE664-25UG
25 μg  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE664-25UG
DescriptionAnti-CBX1 (HP1 beta) Antibody, clone 5E9.1
Alternate Names
  • Chromobox protein homolog 1
  • Heterochromatin protein 1 homolog beta
  • HP1 beta
  • Heterochromatin protein p25
  • M31
  • Modifier 1 protein
Background InformationChromobox protein homolog 1 (UniProt: P83916; also known as Heterochromatin protein 1 homolog beta, HP1 Hsbeta, HP1 beta, Heterochromatin protein p25, M31, Modifier 1 protein, p25 beta) is encoded by the Cbx1 (also known as Cbx) gene (Gene ID: 12412) in human. Chromobox protein homolog 1 is a homodimeric nuclear protein that is a component of heterochromatin. It is expressed in all adult and embryonic tissues. It is essential for formation of and gene repression in heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. Also interacts with lamin B receptor (LBR) to contribute to the association of the heterochromatin with the inner nuclear membrane. The human HP1 homolog CBX1 preferentially recognize H3K9me3 (Peptide binding specificity Kd = 5 μM). However, upon substitution of Threonine 6 to Alanine reduces its affinity significantly (Kd = 45 mM). (Ref.: Kaustov, L., et al. (2011). J. Biol. Chem. 286(1): 521-529).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-CBX1 (HP1 beta), clone 5E9.1 Antibody, Cat. No. MABE664-25UG, is a highly specific mouse monoclonal antibody, that targets CBX1 and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST/His-tagged recombinant protein fragment corresponding to 60 amino acids from the internal region of human Chromobox protein homolog 1 (CBX1).
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5E9.1 specifically detects human Chromobox protein homolog 1 (CBX1). It targets an epitope with in 60 amino acids from the internal region.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CBX1
  • CBX
Purification MethodProtein G purified
UniProt Number
Molecular Weight23 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected CBX1 (HP1 beta) in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABE664-25UG 04054839456206