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MABT1353-100UG Anti-C-Nap1 (Cep250) Antibody, clone 150-230-1

Anti-C-Nap1 (Cep250), clone 150-230-1, Cat. No. MABT1353, is a highly specific mouse monoclonal antibody that targets Centrosome-associated protein CEP250 and has been tested for use in Immunofluorescence and Western Blotting.

MABT1353-100UG
100 μg  
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Áttekintés

Replacement Information
Description
Catalogue NumberMABT1353-100UG
DescriptionAnti-C-Nap1 (Cep250) Antibody, clone 150-230-1
Alternate Names
  • Centrosome-associated protein CEP250
  • 250 kDa centrosomal protein
  • Cep250
  • Centrosomal Nek2-associated protein 1
  • C-Nap1
  • Centrosomal protein 2
Background InformationCentrosome-associated protein CEP250 (UniProt: Q9BV73; also known as 250 kDa centrosomal protein, Cep250, Centrosomal NEK2-associated protein 1, C-Nap1, Centrosomal protein 2) is encoded by the CEP250 (also known as CEP2, CNAP1) gene (Gene ID: 11190) inn human. Cep250 is a component of the core centrosome that is ubiquitously expressed. It can exist as a monomer and homodimer. It specifically associates with the proximal ends of both mother and daughter centrioles during interphase and in mitotic cells it dissociates from the mitotic spindle poles. At the end of cell division, it is shown to re-accumulate at centrosomes. Cep250 is differentially phosphorylated during cell cycle and phosphorylation status regulates its association/dissociation from centrosome. During M phase its C-terminal part is shown to be phosphorylated by NEK2. Two isoforms of Cep250 have been described that are produced by alternative splicing. Loss of Cep250-mediated centriole cohesion leads to an altered cell migration phenotype.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-C-Nap1 (Cep250), clone 150-230-1, Cat. No. MABT1353, is a highly specific mouse monoclonal antibody that targets Centrosome-associated protein CEP250 and has been tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Immunofluorescence
  • Western Blotting
Application NotesImmunofluorescence Analysis: A representative lot detected C-Nap1 (Cep250) in Immunofluorescence applications (Fava, L.L., et. al. (2017). Genes Dev. 31(1):34-45).

Western Blotting Analysis: A representative lot detected C-Nap1 (Cep250) in HeLa S3 cell lysates. (Courtesy of Prof. Erich A. Nigg, Ph.D., Biozentrum, University of Basel, Switzerland).
Biological Information
ImmunogenRecombinant fragment corresponding to 455 amino acids from the C-terminal region of human Centrosome-associated protein CEP250.
Clone150-230-1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 150-230-1 is a mouse monoclonal antibody that detects human Centrosome-associated protein CEP250 (Cep20). It targets an epitope within 455 amino acids from the C-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CEP250
  • CEP2
  • CNAP1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~280 kDa observed; 281.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABT1353-100UG 04054839495991