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MAB131884-25UL Anti-BASE-tag Antibody, clone 7C7

Anti-BASE-tag, clone 7C7, Cat. No. MAB131884, is a mouse monoclonal antibody that recognizes the epitope QPAKKRLNFGQTGD and has been tested for use in Western Blotting, Immunoprecipitation, and Immunofluorescence.

Anti-BASE-tag Antibody, clone 7C7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Cap UniProt Number: Q6JC40
MAB131884-25UL
25 µL  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
VirusIF, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB131884-25UL
DescriptionAnti-BASE-tag Antibody, clone 7C7
Alternate Names
  • Capsid protein VP1
  • Capsid protein AVV9
Background InformationCapsid protein VP1 (UniProt: Q6JC40) is encoded by the Cap gene in Adeno-associated virus 9 (AAV9). Adeno-associated viruses (AAV) are small non-enveloped DNA viruses that can serve as promising viral vectors for gene delivery, but can also elicits cellular and humoral immune responses. AAVs are reported to be nonpathogenic in humans and animals and display low immunogenicity. Following their attachment to cell surface receptor they are internalized via endocytosis and their intracellular trafficking is a rate-limiting, slow and inefficient process. AAVs contain an icosahedral capsid, which is 22 to 26 nm in diameter. They are shown to have a single-stranded DNA genome of approximately 4.7 kilobases that is flanked by two T-shaped inverted terminal repeats (ITRs). The 7C7 an epitope-tag antibody recognizes a basic ten amino acid epitope (QPAKKRLNFGQTGD) with high specificity. The basic nature of the BASE-tag epitope sequence distinguishes it from other commonly used epitope tags, such as Myc, EQKLISEEDL, FLAG, DYKDDDDK, HA, YPYDVPDYA, V5 etc. Clone 7C7 recognizes capsid proteins VP1 and VP2, but not VP3. Based on sequence identity of the epitope sequence, this antibody is expected to recognize VP1 and VP2 of AAV serotypes 1,2,3,6,7,8, and 9. (Ref.: Nonnenmacher, M., and Weber, T (2012). Gene Ther. 19(6); 649-658).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-BASE-tag, clone 7C7, Cat. No. MAB131884, is a mouse monoclonal antibody that recognizes the epitope QPAKKRLNFGQTGD and has been tested for use in Western Blotting, Immunoprecipitation, and Immunofluorescence.
Key Applications
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation: A representative lot immunoprecipitated EGFP-BASE-tag-Actin in HEK293T cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York).
Immunofluorescence: A representative lot detected EGFP-BASE-tag-Actin in HeLa cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York).
Biological Information
ImmunogenEmpty Adeno-associated virus 9 (AAV9) Capsids
Clone7C7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 7C7 is a mouse monoclonal antibody that detects various serotypes of Adeno-associated virus (AVV). It targets an epitope with in 14 amino acids from the N-terminal region.
IsotypeIgG2bκ
Species Reactivity
  • Virus
Species Reactivity NoteVirus (Adenovirus)
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Cap
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cells transfected with GFP-7C7-Actin.

Western Blotting Analysis: A 1:500 dilution of this antibody detected BASE-tag in HEK293 cells transfected with GFP-7C7-Actin.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MAB131884-25UL 04054839563386