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PC742 Anti-Aurora A Rabbit pAb

This Anti-Aurora A Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Aurora A.

Anti-Aurora A Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Aurora-2, Anti-Serine/Threonine Kinase 15, Anti-STK15

PC742
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
Rb
Description
OverviewRecognizes the ~48 kDa Aurora A protein in HEK293 and BT474 cells.

This product has been discontinued.
Catalogue NumberPC742
Brand Family Calbiochem®
SynonymsAnti-Aurora-2, Anti-Serine/Threonine Kinase 15, Anti-STK15
Application Data
Detection of human aurora A by immunoblotting. Samples: Whole cell lysate from SKBr3 cells (figure A) and immunoprecipitation followed by imunnoblot with AsPC-1 cells (figure B). Primary antibody: Anti-Aurora A Rabbit pAb (Cat. No. PC742) (1:20,000). Detection: chemiluminescence.
References
ReferencesCheetham, G.M., et al. 2002. J. Biol. Chem. 277, 42419.
Chen, S.S., et al. 2002. EMBO J. 21, 4491.
Zhou, H., et al. 1998. Nat. Genet. 20, 189.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Positive controlSKBr3 cells, ASPC-1 cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (0.08 µg/ml)
Immunoprecipitation (5 µl/250 µg total protein)
Paraffin Sections (1:250-1:1000)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids encoded within exon 5 of human Aurora A
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
PC742 0

Documentation

Anti-Aurora A Rabbit pAb Certificates of Analysis

TitleLot Number
PC742

References

Hivatkozások áttekintése
Cheetham, G.M., et al. 2002. J. Biol. Chem. 277, 42419.
Chen, S.S., et al. 2002. EMBO J. 21, 4491.
Zhou, H., et al. 1998. Nat. Genet. 20, 189.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-October-2007 RFH
SynonymsAnti-Aurora-2, Anti-Serine/Threonine Kinase 15, Anti-STK15
ApplicationImmunoblotting (0.08 µg/ml)
Immunoprecipitation (5 µl/250 µg total protein)
Paraffin Sections (1:250-1:1000)
Application Data
Detection of human aurora A by immunoblotting. Samples: Whole cell lysate from SKBr3 cells (figure A) and immunoprecipitation followed by imunnoblot with AsPC-1 cells (figure B). Primary antibody: Anti-Aurora A Rabbit pAb (Cat. No. PC742) (1:20,000). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~48 kDa Aurora-A protein.
BackgroundAurora-A is a serine/threonine kinase which has been reported to interact with p53. Overexpression of Aurora-A leads to centrosome amplification, chromosomal instability, and transformation.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids encoded within exon 5 of human Aurora A
IsotypeIgG
Specieshuman
Positive controlSKBr3 cells, ASPC-1 cells
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling
ReferencesCheetham, G.M., et al. 2002. J. Biol. Chem. 277, 42419.
Chen, S.S., et al. 2002. EMBO J. 21, 4491.
Zhou, H., et al. 1998. Nat. Genet. 20, 189.