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MABN638-I-25UG Anti-Amyloid beta fibrils Antibody, clone M87

Anti-Amyloid beta fibrils, clone M87, Cat. No. MABN638-I, is a highly specific rabbit recombinant monoclonal antibody that targets Amyloid beta fibrils and has been tested for use in Immunohistochemistry (Paraffin).

MABN638-I-25UG
25 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberMABN638-I-25UG
DescriptionAnti-Amyloid beta fibrils Antibody, clone M87
Alternate Names
  • Amyloid-beta precursor protein
  • APP
  • ABPP
  • APPI
  • Alzheimer disease amyloid protein
  • Amyloid precursor protein
  • Amyloid-beta A4 protein
  • Cerebral vascular amyloid peptide
  • CVAP
  • PreA4
  • Protease nexin-II
  • PN-II
Background InformationAmyloid-beta precursor protein (UniProt: P05067; also known as APP, ABPP, APPI, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta A4 protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. Under normal conditions A4 functions as a cell surface receptor and performs physiological functions relevant to neurite growth, neuronal adhesion and axonogenesis. APP undergoes extensive post-translational modification including glycosylation, phosphorylation, and tyrosine sulfation, as well as many types of proteolytic processing to generate peptide fragments. APP is proteolytically processed under normal cellular conditions by alpha-secretase or beta-secretase to generate and release soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. In Alzheimer s disease processing of C99 generates amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42) that form amyloid plaques. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. They bind transient metals such as copper, zinc and iron. Under pathological conditions Amyloid beta A4 protein is the precursor for the disease associated proteins, beta amyloid 1-40, and 1-42, major components of plaques in Alzheimer s disease. Clone M87 recognizes a specific conformation of residues 3-7 of the amyloid beta peptide sequence (EFRHD) found in amyloid fibrils. It does not recognize pyroglutamyl Ab at residue 3. It also reacts with IAPP and alpha-synuclein fibrils but not monomers, indicating that it recognizes a generic, sequence independent epitope. (Ref.: Nussbaum, JM., et al. (2012). Nature. 485(7400); 651-655; McLean, D., et al. (2013) ACS Chem Neurosci. 4, 613-623).
References
Product Information
FormatPurified
PresentationPurified rabbit recombinant monoclonal antibody in PBS without preservatives.
Applications
ApplicationAnti-Amyloid beta fibrils, clone M87, Cat. No. MABN638-I, is a highly specific rabbit recombinant monoclonal antibody that targets Amyloid beta fibrils and has been tested for use in Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Amyloid beta fibrils in APP mouse brain tissue sections.
Biological Information
ImmunogenFibrillar Abeta 1-42 peptide
CloneM87
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityClone M87 is a conformation-sensitive rabbit recombinant monoclonal antibody that detects Amyloid beta fibrils. It recognizes a specific conformation of residues 3-7 of the amyloid beta peptide sequence (EFRHD) found in amyloid fibrils.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeRecombinant Monoclonal Antibody
Gene Symbol
  • APP
  • A4
  • AD1
Purification MethodProtein A purified
UniProt Number
Molecular Weight86.94 kDa calculated for monomeric and ~ 300 kDa for aggregated form.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human Alzheimer's diseased brain tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Amyloid beta fibrils in human Alzheimer's diseased brain tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABN638-I-25UG 04061841236139