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04-642-I-100UG Anti-Ago2 Antibody, clone 9E8.2

Anti-Ago2, clone 9E8.2, Cat. No. 04-642-I, is a mouse monoclonal antibody that detects of Protein argonaute-2 (Ago2) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

04-642-I-100UG
100 μg  
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Áttekintés

Replacement Information
Description
Catalogue Number04-642-I-100UG
DescriptionAnti-Ago2 Antibody, clone 9E8.2
Alternate Names
  • Protein argonaute-2
  • Argonaute2
  • hAgo2
  • Argonaute RISC catalytic component 2
  • Eukaryotic translation initiation factor 2C
  • eIF-2C
  • eIF2C
  • PAZ Piwi domain protein
  • PPD
  • Protein slice
Background InformationProtein argonaute-2 (UniProt: Q9UKV8; also known as Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C, eIF-2C, eIF2C, PAZ Piwi domain protein, PPD, Protein slicer) is encoded by the AGO2 (also known as EIF2C2) gene (Gene ID: 27161) in human. Ago2 is a highly conserved member of the AGO family of proteins with catalytic activity and is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by Ago2 and binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation and is independent of endonuclease activity. Ago2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. Ago2 is shown to have four core domains that are known as N domain, PAZ domain (aa 235-348), MID domain, and PIWI domain (aa 517-818). The PAZ domain is an RNA binding module that recognizes the 3 end of both siRNA and miRNA, in a sequence independent manner and is reported to be strictly required for Ago2 to form the RISC complex. The PIWI domain has major roles within Ago2, including RNase H like endonucleolytic activity and regulating the interaction between MID domain and DNA/RNA. Ago2 is shown to undergo 4-hydoxylation that enhances its stability but is not essential for miRNA binding or endonuclease activity.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Ago2, clone 9E8.2, Cat. No. 04-642-I, is a mouse monoclonal antibody that detects of Protein argonaute-2 (Ago2) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Ago2 in Immunoprecipitation applications (Benhamed, M., et. al. (2012). Nat Cell Biol. 14(3):266-75; Pandolfini, L., et. al. (2016). Genome Biol. 17(1):94).

Western Blotting Analysis: A representative lot detected Ago2 in Western Blotting applications (Janowski, B.A., et. al. (2006). Nat Struc Mol Biol. 13(9):787-92; Sen, G.L., et. al. (2005). Nat Cell Biol. 7(6):633-6).

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Ago2 in A431 cells.

Immunocytochemistry Analysis: A representative lot detected Ago2 in Immunocytochemistry applications (Sen, G.L., et. al. (2005). Nat Cell Biol. 7(6):633-6).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human Protein argonaure-2 (Ago2).
EpitopeN-terminal
Clone9E8.2
Concentration1.0 ug/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 9E8.2 is a mouse monoclonal antibody that detects Protein argonaute-2 (Ago2). It targets an epitope within 19 amino acids from the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • AGO2
  • EIF2C2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~97 kDa observed; 97.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago2 in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
04-642-I-100UG 04065265397995