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NE1041 Anti-AGT Mouse mAb (1A10)

Anti-AGT Mouse mAb (1A10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Angiotensinogen

NE1041
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~50 kDa AGT protein in HepG2 cells.
Catalogue NumberNE1041
Brand Family Calbiochem®
SynonymsAnti-Angiotensinogen
Application Data
Detection of human AGT by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AGT Mouse mAb (1A10) (Cat. No. NE1041) (1-5 µg/ml). Detection: chemiluminescence.
References
ReferencesJain S., et al. 2008. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295, R1849.
Jain, S., et al. 2007. Am. J. Physiol. Cell Physiol. 293, C401.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlHepG2 cells
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 376-485 human AGT, expressed as a GST fusion protein
Clone1A10
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
NE1041 0

Documentation

References

Hivatkozások áttekintése
Jain S., et al. 2008. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295, R1849.
Jain, S., et al. 2007. Am. J. Physiol. Cell Physiol. 293, C401.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-Angiotensinogen
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human AGT by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AGT Mouse mAb (1A10) (Cat. No. NE1041) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~50 kDa AGT protein.
BackgroundThe protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 376-485 human AGT, expressed as a GST fusion protein
Clone1A10
IsotypeIgG₁
Specieshuman
Positive controlHepG2 cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesJain S., et al. 2008. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295, R1849.
Jain, S., et al. 2007. Am. J. Physiol. Cell Physiol. 293, C401.