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ST1531 Anti-ADH4 Mouse mAb (1D2)

Anti-ADH4 Mouse mAb (1D2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ST1531
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~37 kDa ADH4 protein in human liver tissue and HeLa NE cells.
Catalogue NumberST1531
Brand Family Calbiochem®
Application Data
Detection of human ADH4 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-ADH4 Mouse mAb (1D2) (Cat. No. ST1531) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human ADH4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-ADH4 Mouse mAb (1D2) (Cat. No. ST1531) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human ADH4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human liver tissue. Primary antibody: Anti-ADH4 Mouse mAb (1D2) (Cat. No. ST1531) (3 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHuman liver tissue, HeLa NE cells
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 52-151 of human ADH4, expressed as a GST fusion protein
Clone1D2
HostMouse
IsotypeIgG2a
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ST1531 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-November-2010 RFH
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human ADH4 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-ADH4 Mouse mAb (1D2) (Cat. No. ST1531) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human ADH4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-ADH4 Mouse mAb (1D2) (Cat. No. ST1531) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human ADH4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human liver tissue. Primary antibody: Anti-ADH4 Mouse mAb (1D2) (Cat. No. ST1531) (3 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~37 kDa ADH4 protein.
BackgroundADH4 is a class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 52-151 of human ADH4, expressed as a GST fusion protein
Clone1D2
IsotypeIgG2a
Specieshuman
Positive controlHuman liver tissue, HeLa NE cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review