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AP1144 Anti-ACP1 Mouse mAb (4B10)

Anti-ACP1 Mouse mAb (4B10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Acid Phosphatase of Erythrocyte

AP1144
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~17 kDA ACP1 protein in HeLa, PC-12, and NIH3T3 cells.
Catalogue NumberAP1144
Brand Family Calbiochem®
SynonymsAnti-Acid Phosphatase of Erythrocyte
Application Data
Detection of human ACP1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-ACP1 Mouse mAb (4B10) (Cat. No. AP1144) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human ACP1 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-ACP1 Mouse mAb (4B10) (Cat. No. AP1144) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human ACP1 by immunoblotting. Sample: Whole cell lysate from NIH 3T3 cells. Primary antibody: Anti-ACP1 Mouse mAb (4B10) (Cat. No. AP1144) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells, PC-12 cells, NIH3T3 cells
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant ACP1 (aa1-159), expressed as a GST fusion protein
Clone4B10
HostMouse
IsotypeIgG2,b
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
AP1144 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-October-2010 JLN
SynonymsAnti-Acid Phosphatase of Erythrocyte
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human ACP1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-ACP1 Mouse mAb (4B10) (Cat. No. AP1144) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human ACP1 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-ACP1 Mouse mAb (4B10) (Cat. No. AP1144) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human ACP1 by immunoblotting. Sample: Whole cell lysate from NIH 3T3 cells. Primary antibody: Anti-ACP1 Mouse mAb (4B10) (Cat. No. AP1144) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionA purified mouse monoclonal antibody, which recognizes the ~17 kDA ACP1 protein.
BackgroundPhosphatase enzymes catalyse hydrolysis of phosphoric acid esters of various alcohols.
HostMouse
ImmunogenFull-length, recombinant ACP1 (aa1-159), expressed as a GST fusion protein
Clone4B10
IsotypeIgG2,b
Specieshuman, mouse, rat
Positive controlHeLa cells, PC-12 cells, NIH3T3 cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review