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MABS416 Anti-ABCG5, clone 1B5E10 Antibody

MABS416
100 µL  
Purchase on Sigma-Aldrich

Különleges ajánlatok

Áttekintés

Replacement Information

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Kulcsspecifikációk táblázata

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, FC, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS416
ReplacesMABN1076
DescriptionAnti-ABCG5, clone 1B5E10 Antibody
Alternate Names
  • ATP-binding cassette sub-family G member 5
  • Sterolin-1
  • ABCG5
Background InformationATP-binding cassette sub-family G member 5, also known as Sterolin-1, and encoded by the gene name ABCG5, plays an important role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. ABCG5 is localized too the membrane and is expressed in the liver, lower levels in the small intestine and colon. Mutations associated with ABCG5 is Sitosterolemia, which is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal in PBS containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ABCG5, clone 1B5E10 Antibody is an antibody against ABCG5 for use in western blotting, IHC, flow cytometry & ELISA.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Flow Cytometry
  • ELISA
Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ABCG5 in cervical and rectum cancer tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected ABCG5 in A549 cells.

ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Biological Information
ImmunogenPurified recombinant fragment of human ABCG5 expressed in E. Coli
Clone1B5E10
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • ABCG5
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~70 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HL7702, Raji and Jurkat cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ABCG5 in HL7702, Raji and Jurkat cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABS416 04053252957796