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MABE317 Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01

Detect 5-Hydroxymethylcytosine (5hmC) using this Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 validated for use in Dot Blot, ICC, Immunoprecipitation.

Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology
MABE317
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
ADB, ICC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE317
DescriptionAnti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01
Alternate Names
  • 5hmeC
  • 5' hydroxymethyl cytosine
  • 5'OH methyl cytosine
  • 5'OHmeC
Background Information5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect 5-Hydroxymethylcytosine (5hmC) using this Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 validated for use in Dot Blot, ICC, Immunoprecipitation.
Key Applications
  • Dot Blot
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesHydroxymethylated DNA Immunoprecipitation (hMeDIP) Analysis: A representative lot was used by an independent laboratory to immunoprecipitate 5-Hydroxymethylcytosine DNA usingh chomatin from 293T cells overexpressing mTET1-CD. (Dr. D. Chen, Shanghai Institute of Biological Sciences)

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to stain 5-Hydroxymethylcytosine DNA in cortical neurons from atg5-disrupted mouse. (Dr. D. Chen, Shanghai Institute of Biological Sciences)
Biological Information
ImmunogenBSA-conjugated recombinant Hydroxymethylcytosine DNA.
CloneHMC-MA01
HostMouse
SpecificityThis antibody detects 5-Hydroxymethylcytosine, but not 5-Methylcytosine or unmethylated cytosine.
IsotypeIgG1κ
Species Reactivity
  • All
Species Reactivity NoteExpected to detect 5-Hydroxymethylcytosine in all species
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Dot Blot in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-Hydroxymethylcytosine DNA.

Dot Blot Analysis: A 1:5000 dilution of this antibody detected only 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
MABE317 04053252551857

Documentation

Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 MSDS

Title

Safety Data Sheet (SDS) 

Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 Certificates of Analysis

TitleLot Number
Anti-5-Hydroxymethylcytosine (5hmC), clone HMC-MA01 2930991
Anti-5-Hydroxymethylcytosine (5hmC), clone HMC-MA01 - 2458017 2458017
Anti-5-Hydroxymethylcytosine (5hmC), clone HMC-MA01 - 3503874 3503874
Anti-5-Hydroxymethylcytosine (5hmC), clone HMC-MA01 - 3760035 3760035
Anti-5-Hydroxymethylcytosine (5hmC), clone HMC-MA01 - Q2063315 Q2063315
Anti-5-Hydroxymethylcytosine (5hmC), clone HMC-MA01 Monoclonal Antibody 3113128

Brochure

Title
CpGenome™ 5-hmC Quantitation Kit