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15-115 AMPK Pathway Explorer Antibody MiniPack

This Antibody pack contains Anti-AMPK α1 Antibody, Anti-AMPKα2 Antibody, Anti-phospho-AMPKα Antibody(Thr172).

AMPK Pathway Explorer Antibody MiniPack MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling, Apoptosis & Cancer Research Sub-Category: Insulin/Energy Signaling Gene Symbol: AMPK UniProt Number: Q13131
15-115
3 vials/Pk  
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Description
Catalogue Number15-115
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAMPK Pathway Explorer Antibody MiniPack
Alternate Names
  • AMP-Activated Protein Kinase
Background InformationPathway Explorer Antibody MiniPack:
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).

AMPK:
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.

*See full size versions for corresponding references.
References
Product Information
Components
  • 07-350SP Anti-AMPK α1; 60 µg
  • 07-363SP Anti-AMPKα2; 60 µg
  • 07-681SP Anti-phospho-AMPKα (Thr172); 60 µg
FormatPurified
Control
  • For 07-681SP use HEK293 cells or rat liver AMPK.
  • For 07-363SP use Rat liver preparation.
  • For 07-350SP use A431cell lysate, or lysate of HeLa cells treated with heat shock.
Presentation3 individual tubes containing either Anti-AMPK α1; Anti-AMPKα2; or Anti-phospho-AMPKα (Thr172)
PropertiesEach vial is 30% the size of the parent catalog number
Quality LevelMQ100
Applications
ApplicationThis Antibody pack contains Anti-AMPK α1 Antibody, Anti-AMPKα2 Antibody, Anti-phospho-AMPKα Antibody(Thr172).
Key Applications
  • Western Blotting
Biological Information
ImmunogenVaries. See individual Data Sheets
Epitope07-350SP and 07-363SP: Internal
Concentration1 mg/mL
HostRabbit
Specificity07-681SP: AMPKα phosphorylated on Thr172.

07-363SP: AMP-Activated Protein Kinase alpha 2 (AMPKα2).

07-350SP: AMPKα1
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity Note07-681SP:Human and rat. Expected to cross-react with mouse based on 100% sequence homology.

07-363SP: Human and rat.

07-350SP: Human, rat, and mouse.
Antibody TypePolyclonal Antibody
Cross ReactivityVaries. See individual Data Sheets
Entrez Gene Number
Entrez Gene SummaryAMPK: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene Symbol
  • AMPK
Modifications
  • Phosphorylation
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Ref.8 Ref.9

Catalytic activity ATP + a protein = ADP + a phosphoprotein.

Cofactor Magnesium.

ENZYME REGULATION: Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. Ref.8 Ref.9

SUBUNIT STRUCTURE: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1 and FNIP2. Ref.14 Ref.15

SEQUENCE SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Contains 1 protein kinase domain.

SEQUENCE CAUTION: The sequence AAH48980.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened
Molecular Weight~63 kDa;
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance07-681SP:Routinely evaluated by immunoblot on RIPA lysates from overnight starved HEK293 cells and partially purified rat liver AMPK.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected phospho-AMPK α in RIPA lysates from overnight serum starved HEK293 cells and partially purified rat liver AMPK. Signal was reduced after treatment of lysate with λ-phosphatase.

07-363SP: Routinely evaluated by immunoblot in a rat liver preparation.

Western Blot Analysis:
1-2 μg/mL of this lot detected AMPK α2 in a rat liver preparation.


07-350SP: Routinely evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Packaging Information
Material Size3 vials/Pk
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
15-115 04053252005527

Documentation

AMPK Pathway Explorer Antibody MiniPack MSDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
The influence of carbohydrate-protein co-ingestion following endurance exercise on myofibrillar and mitochondrial protein synthesis.
Leigh Breen,Andrew Philp,Oliver C Witard,Sarah R Jackman,Anna Selby,Ken Smith,Keith Baar,Kevin D Tipton
The Journal of physiology  589  2010

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