Chromolith® HPLC Columns Speed of Analysis |
This combination results in separations which are twice as fast as conventional 5 μm particulate columns – at half the back pressure. Furthermore, flow rates can be easily varied from 1 mL up to 9 mL per minute, with the same high-quality resolution. Besides rapid separations, Chromolith® HPLC columns also ensure fast re-equilibration between analyses.
High-Speed Separation of Five Beta-Blockers
A mixture of five beta-blocker drugs demonstrates the extreme time savings and high separation efficiency made possible with Chromolith® columns. Due to the excellent mass transfer properties of the monolithic skeleton, high-speed separation is possible even at a high flow rate. The beta-blockers were well separated with excellent peak symmetry. At 9 mL/min, the analysis time was less than one minute, and the column back pressure was only 153 bar.
Application Example
| Column | Chromolith® Performance RP-18 endcapped, 100-4.6 mm | ||
|---|---|---|---|
| Mobile phase |
Isocratic acetonitrile / 0.1% trifluoroacetic acid in water, 20/80 (v/v) |
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|
| Pressure | Total pressure (including HPLC system) 25°C | ||
| Detection | UV 220 nm | ||
| Injection volume |
5 μL | ||
| Sample | Atenolol 63 μg/mL Pindolol 29 μg/mL Metoprolol 108 μg/mL Celiprolol 104 μg/mL Bisoprolol 208 μg/mL |
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