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70174 pT7Blue Blunt Vector (linearized) - Novagen

pT7Blue Blunt Vector (linearized) - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70174
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概述

Replacement Information

价格及供货情况

产品目录编号 库存情况包装 数量 / 包装 价格 数量
70174-3CN
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      		 塑胶安瓿;塑胶针药瓶 20 rxn
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      70174-4CN
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          		 塑胶安瓿;塑胶针药瓶 40 rxn
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          Description
          Overview

          The pT7Blue vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an EcoR V site used for T-cloning flanked by an Nde I site, which allows inserts to be conveniently subcloned into the Nde I site of many pET vectors.
          Catalogue Number70174
          Brand Family Novagen®
          Features and benefits
          • No restriction enzymes or special primers
          • Compatible with any DNA polymerase
          • Independent of 3ʹ-dA addition or sequence context of DNA ends
          • Blue/white screening with seven different vectors, including pETBlue expression vectors
          • Simple protocol takes less than one hour* from PCR product to plating transformants

          * All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth.
          References
          References

          Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.
          Product Information
          Components
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          产品目录编号 GTIN
          70174-3CN 04055977258363
          70174-4CN 04055977258370

          Documentation

          pT7Blue Blunt Vector (linearized) - Novagen 分析证书

          标题批号
          70174

          参考

          参考信息概述

          Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.

          引用

          标题
        • Ekaterina Bogdanova, et al. (2008) Transcription regulation of the type II restriction-modification system AhdI. Nucleic Acids Research 36, 1429-1442.
        • Miho Hirabayashi, et al. (2005) Transformation of skeletal muscle from fast- to slow-twitch during acquisition of cold tolerance in the chick. Endocrinology 146, 399-405.
        • J. K. Jang, T. Rahman and K. S. McKim. (2005) The kinesinlike protein subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. 16, 4684-4694.
        • Rahul Pal, et al. (2005) Evidence for multiple shared antigenic determinants within Ro60 and other lupus-related ribonucleoprotein autoantigens in human autoimmune responses. Journal of Immunology 175, 7669-7677.
        • Dana R. Rundle, et al. (2004) Myristoyl-CoA: protein N-myristoyltransferases: isoform identification and gene expression in retina. Molecular Vision 10, 177-185.
          		•

          用户协议

          标题
          TB053 Academic and Non-profit Laboratory Assurance Letter
          TB183 Perfectly Blunt® Cloning Kits

          载体图

          标题
          TB017VM pT7Blue Vector Map

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          种类

          Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Vectors