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A cell-permeable neomycin derived artificial nucleobase conjugate. Inhibits the production of oncogenic miRNAs 372 (IC₅₀ = 2.4 µM) by binding to pre-miRNAs and interrupts processing by Dicer.
More>>A cell-permeable neomycin derived artificial nucleobase conjugate. Inhibits the production of oncogenic miRNAs 372 (IC₅₀ = 2.4 µM) by binding to pre-miRNAs and interrupts processing by Dicer. Less<<
miR-372/3 Inhibitor, Neomycin-S - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
A cell-permeable neomycin derived artificial nucleobase conjugate that inhibits the production of oncogenic miRNAs 372 (IC50 = 2.4 µM; Kd = 16 nM) and 373 (IC50 = 5.13 µM Kd = 21.4 nM) by directly binding to their precursors pre-miRNAs and interrupts their processing by Dicer. In comparison, neomycin does not affect these miRNAs (IC50 = 125 µM; Kd = 7.4 µM). Shown to depress proliferation of AGS gastric cancer cells expressing miRNA 372 and 373. However, it has no effect on MKN74 gastric epithelial cells that do not express miRNA 372 and 373. Reduced levels of miRNA 372 results in de-repression of their targets, such as the cell cycle regulator large tumor suppressor homologue 2 (LATS2).
Please note that the molecular weight for this compound is batch-specific due to variable water content.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
20-June-2014 JSW
Synonyms
miRNA-372 Inhibitor, miRNA-373 Inhibitor
Description
A cell-permeable neomycin derived artificial nucleobase conjugate that inhibits the production of oncogenic miRNAs 372 (IC50 = 2.4 µM; Kd = 16 nM) and 373 (IC50 = 5.13 µM Kd = 21.4 nM) by directly binding to their precursors pre-miRNAs and interrupts their processing by Dicer. In comparison, neomycin does not affect these miRNAs (IC50 = 125 µM; Kd = 7.4 µM). Shown to depress proliferation of AGS gastric cancer cells expressing miRNA 372 and 373. However, it has no effect on MKN74 gastric epithelial cells that do not express miRNA 372 and 373. Reduced levels of miRNA 372 results in de-repression of their targets, such as the cell cycle regulator large tumor suppressor homologue 2 (LATS2).
Form
Off-white solid
Intert gas (Yes/No)
Packaged under inert gas
Chemical formula
C₃₉H₆₀O₁₄N₁₂S
Purity
≥98% by HPLC
Solubility
DMSO (100 mg/ml)
Storage
Protect from light
≤ -70°C
Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-2-°C). Stock solutions are stable for up to 3 months at -20°C.
