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420220 Sigma-AldrichiPSC Induction Enhancer, Thiazovivin - CAS 1226056-71-8 - Calbiochem

iPSC Induction Enhancer, Thiazovivin, CAS 1226056-71-8, is a cell-permeable ROCK inhibitor that prevents trypsinization-induced apoptosis in hESC cultures.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: N-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII

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概述

Replacement Information

重要规格表

CAS #	Empirical Formula
1226056-71-8	C₁₅H₁₃N₅OS

价格及供货情况

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Description
Overview	A cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) as a ROCK inhibitor (71% inhibition at 2 µM). Effectively prevents trypsinization-induced apoptosis in hESC cultures by stabilizing newly synthesized cell surface E-cadherin and cell-cell adhesion. Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated hESCs even in the absence of ECM. Greatly augments SB431542 (Cat. No. 616461) and PD0325901 (Cat. No. 444966) in improving iPSCs generation efficiency from 4-TF- (Oct44, Klf4, Sox2, and c-Myc) transfected fibroblast cultures.
Catalogue Number	420220
Brand Family	Calbiochem®
Synonyms	N-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII

References
References	Xu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129 Lin, T., et al. 2009. Nat. Methods 6, 805.

Product Information
CAS number	1226056-71-8
Form	Tan powder
Hill Formula	C₁₅H₁₃N₅OS
Chemical formula	C₁₅H₁₃N₅OS
Structure formula Image
Quality Level	MQ100

Applications
Application	iPSC Induction Enhancer, Thiazovivin, CAS 1226056-71-8, is a cell-permeable ROCK inhibitor that prevents trypsinization-induced apoptosis in hESC cultures.

Biological Information
Purity	≥98% by HPLC

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Protect from Light	Protect from light
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Packaging Information
Packaged under inert gas	Packaged under inert gas

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
420220-10MGCN	04055977210811

Documentation

iPSC Induction Enhancer, Thiazovivin - CAS 1226056-71-8 - Calbiochem MSDS

职位
物料安全数据表 (MSDS)

iPSC Induction Enhancer, Thiazovivin - CAS 1226056-71-8 - Calbiochem 分析证书

标题	批号
420220	输入批号

参考

参考信息概述
Xu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129 Lin, T., et al. 2009. Nat. Methods 6, 805.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	17-April-2012 JSW
Synonyms	N-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII
Description	A cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) in inhibiting ROCK enzymatic activity (71% inhibition at 2 µM). Single-cell dissociation of cultured hESCs is demonstrated to hyperactivate Rho-ROCK signaling and result in cell death, while suppression of such hyperactivation by Tzv (2 µM) or Y-27632 (2 µM) stabilize newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion and prevents trypsinization-induced apoptosis (12% vs 81% TUNEL-positive cells after trypsinization in matrigel cultures with 2 µM Tzv or DMSO, respectively). Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated individual hESCs even in the absence of ECM (24% vs 90% TUNEL-positive cells after trypsinization in non-coating plates with 2 µM Tzv or Ptn, respectively). Simultaneous blockage of TGFβ and MEK-ERK pathways by 2 µM SB431542 (Cat. No. 616461) and 0.5 µM PD0325901 (Cat. No. 444966) is reported to result in >50-fold improvement of human iPSCs (induced Pluripotent Stem Cells) generation from fibroblast cultures virally transfected with 4-TF (Oct44, Klf4, Sox2, and c-Myc), while the inclusion of 0.5 µM Tzv on top of SB431542 and PD0325901 is demonstrated to further improve the efficiency to an overall improvement of >200-fold from 4-TF-induction alone.
Form	Tan powder
Intert gas (Yes/No)	Packaged under inert gas
CAS number	1226056-71-8
Chemical formula	C₁₅H₁₃N₅OS
Structure formula
Purity	≥98% by HPLC
Solubility	DMSO (100 mg/ml)
Storage	Protect from light +2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity	Standard Handling
References	Xu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129 Lin, T., et al. 2009. Nat. Methods 6, 805.

种类

Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Stem Cell Biology

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