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14-733 Sigma-Aldrichc-Kit (V654A) Protein, active, 10 µg

V654A is an acquired resistance mutation found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance & rapid progession of GISTs.

c-Kit (V654A) Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

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概述

Replacement Information

Description
Catalogue Number	14-733
Brand Family	Chemicon®
Trade Name	Chemicon
Description	c-Kit (V654A) Protein, active, 10 µg
Overview	N-terminal GST tagged, recombinant human cKit, amino acids 544 - end containing the V654A mutation. Co-ordinates encompass the cytoplasmic domain (544-976) according in Swiss Prot P10721. V654A is an acquired resistance mutation which has been found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance and rapid progession of GISTs. (Chen LL et al., Cancer Research, (2004); 64:5913-5919).

References

Product Information
Quality Level	MQ100

Applications
Application	V654A is an acquired resistance mutation found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance & rapid progession of GISTs.
Key Applications	Kinase Assay

Biological Information
Source	recombinant protein expressed in Sf21 cells
Specific Activity	169 U/mg, where one unit of cKit (V654A) activity is defined as 1nmol phosphate incorporated into 250 μM GGMEDIYFEFMGGKKK per minute at 30 °C with a final ATP concentration of 100 μM.
Entrez Gene Number	NM_000222.2 NM_001093772.1
Entrez Gene Summary	This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene Symbol	KIT C-Kit PBT CD117 SCFR c-kit
Protein Target	c-Kit
Purification Method	Purified using glutathione-agarose. Purity 99% by SDS-PAGE and Coomassie blue staining.
Target Sub-Family	TK
UniProt Number	P10721
UniProt Summary	FUNCTION: SwissProt: P10721 # This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K). SIZE: 976 amino acids; 109865 Da SUBUNIT: Interacts with APS. Interacts with the tenth PDZ domain of MPDZ. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DISEASE: SwissProt: P10721 # Defects in KIT are a cause of piebaldism [MIM:172800]. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. & Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764]. & Defects in KIT have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT). SIMILARITY: SwissProt: P10721 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Molecular Weight	76.7kDa.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by phosphorylation of 250 μM GGMEDIYFEFMGGKKK. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active cKit (V654A).
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap. Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.

Packaging Information
Material Size	10 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
14-733	04053252513480

Documentation

c-Kit (V654A) Protein, active, 10 µg MSDS

职位
物料安全数据表 (MSDS)

c-Kit (V654A) Protein, active, 10 µg 分析证书

标题	批号
Kit (V654A), active - 1643500-B	1643500-B

相关产品和应用

Alternative Packsize

产品目录编号

描述

c-Kit (V654A) Protein, active, 250 µg

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产品族

种类

Life Science Research > Proteins and Enzymes > Purified Kinases

Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases

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