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Recombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
Catalogue Number
662065
Brand Family
Calbiochem®
Synonyms
Me-Ub
References
References
Hershko, A. and Heller, H. 1991. J. Biol. Chem.266, 16376. Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun.128, 1079. Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz.91, 570. Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem.106, 186. Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem.254, 4359.
Hershko, A. and Heller, H. 1991. J. Biol. Chem.266, 16376. Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun.128, 1079. Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz.91, 570. Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem.106, 186. Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem.254, 4359.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
16-September-2008 RFH
Synonyms
Me-Ub
Description
Recombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
Form
Lyophilized solid
Recommended reaction conditions
Typical concentrations for non-rate-limiting support of in vitro conjugation reactions range from 200 µM to 1 mM depending on the reaction conditions.
Purity
≥95% by SDS-PAGE
Solubility
Aqueous buffers (50 mg/ml)
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity
Standard Handling
References
Hershko, A. and Heller, H. 1991. J. Biol. Chem.266, 16376. Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun.128, 1079. Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz.91, 570. Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem.106, 186. Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem.254, 4359.
