Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
A chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry. A trypsin to protein ratio of 1:100 to 1:20 is recommended for mass spectrometry protocols. Note: 1 SET = 5 X 20 µg of modified trypsin, and 1 ml of resuspension buffer.
Catalogue Number
650279
Brand Family
Calbiochem®
References
References
Henzel, W.J., et al. 1993. PNAS90, 5011. Rice, R.H., et al. 1977. Biochim Biophys Acta492, 316..
Product Information
Unit of Definition
One BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.
Chymostrypsin Activity: <3 BTEE U/mg (one BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8.)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R Phrase
R: 36/37/38-42
Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation.
S Phrase
S: 22-24-26-36/37-45
Do not breathe dust. Avoid contact with skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and gloves. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Harmful
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.
Henzel, W.J., et al. 1993. PNAS90, 5011. Rice, R.H., et al. 1977. Biochim Biophys Acta492, 316..
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
18-May-2009 JSW
Description
A chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry.
Form
Lyophilized
Recommended reaction conditions
Materials Provided:
• Trypsin, Porcine, MS Grade (Kit Component No. KP31816-20UG): 5 vials, 20 µg each
• Trypsin Resuspension Buffer (Kit Component No. KP31854-0.5ML): 1 vial, 0.5 ml
Preparation Before Use:• Before opening the trypsin centrifuge the tube at 15,000 x g for 5 min. Suspend the MS Grade Trypsin in the Trypsin-Suspension Buffer. Recommended reconstitution concentration 0.5-1 µg/µl.
IMPORTANT NOTE: In some cases, after reconstitution, there is insoluble material, which is a non-protein and will not interfere with trypsin activity. If you prefer to remove it, centrifuge the tube briefly and transfer the supernatant to a clean tube.
Guidelines for use:• For mass spectrometry sequence analysis, MS Grade Trypsin to protein ratio of 1:20 to 1:100 is recommended.
• Use freshly reconstituted MS Grade Trypsin in any sequence analysis. Any unused trypsin may be stored at -70°C and used within two weeks. Avoid repeated freeze-thaw cycle after re-suspension, which may lead to performance loss.
• Any available protocol can be used for digestion of proteins with MS Grade Porcine Trypsin.
Contaminants
Chymostrypsin Activity: <3 BTEE U/mg (one BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8.)
Specific activity
≥10,000 U/mg
Unit definition
One BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.
Toxicity
Harmful
References
Henzel, W.J., et al. 1993. PNAS90, 5011. Rice, R.H., et al. 1977. Biochim Biophys Acta492, 316..
