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Native troponin I purified from human heart. Inhibitory subunit of troponin that binds to actin in the absence of Ca2+, thus inhibiting the ATPase activity of actomyosin. Presence of hcTnI in plasma has been used for early detection of myocardial infarction. Useful as a standard in immunoassay and as an immunogen for antisera production. Also useful as a tracer for iodination.
Catalogue Number
648480
Brand Family
Calbiochem®
Synonyms
hcTnI
References
References
Falahati, A., et al. 1999. Am. Heart J. 137, 332. Mangano, D.T. 1994. Anesthesiology 81, 1317.
Falahati, A., et al. 1999. Am. Heart J. 137, 332. Mangano, D.T. 1994. Anesthesiology 81, 1317.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
16-April-2008 JSW
Synonyms
hcTnI
Description
Native troponin I purified from human heart. Inhibitory subunit of troponin that binds to actin in the absence of Ca2+, thus inhibiting the ATPase activity of actomyosin. Presence of Tnl in plasma has been used for early detection of myocardial infarction. Useful as a standard in immunoassay and as an immunogen for anti-sera production. Also useful as a tracer for iodination.
Form
Lyophilized
Formulation
Lyophilized from 10 mM HCl.
Source
Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number
77108-40-8
Purity
≥95% by SDS-PAGE
Solubility
Urea/Tris buffer (20 mM Tris, HCl, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol) (1 mg/ml)
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity
Standard Handling
References
Falahati, A., et al. 1999. Am. Heart J. 137, 332. Mangano, D.T. 1994. Anesthesiology 81, 1317.