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595504 Sigma-AldrichThiol Fluorescent Probe IV - CAS 1173888-41-9 - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: 3-(7-Hydroxy-2-oxo-2H-chromen-3-ylcarbamoyl)acrylic acid methyl ester

Recommended Products

概述

Replacement Information

重要规格表

CAS #	Empirical Formula
1173888-41-9	C₁₄H₁₁NO₆

价格及供货情况

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Description
Overview	A cell-permeable, practically non-fluorescent (Φ₀ = 0.001) coumarin cis-acrylate derivative that exhibits a 470-fold increase in fluorescence property (Φ = 0.47; Ex. 400 nm, Em. 465 nm) upon an 1:1 Michael adduct formation with thiol/sulfhydryl and acts as a fast-reacting (t_1/2 = 3 msec with 50 µM probe and 50 mM 2-ME at t = 0), highly sensitive (Detection limit ≤0.5 nM GSH with 100 nM probe), and thiol-specific fluorogenic probe, displaying high reactivity toward 2-ME, GSH, and Cysteine, but not non-thiol-containing GSSG or Cystine. A more sensitive alternative to Coomassie Blue in staining of BSA on SDS-PAGE gels and has been applied successfully in fluorescent labeling cellular thiols in intact HEK293 cells as well as in detecting glutathione reductase activity in cell-free enzymatic assays. ThioGlo^®1 (Cat. No. 595501), ThioGlo^®3, and ThioGlo^®5, in comparison, exhibit much lower fluorescence quantum yield increases upon reaction with thiol (61-, 156-, and 28-fold increase, respectively).
Catalogue Number	595504
Brand Family	Calbiochem®
Synonyms	3-(7-Hydroxy-2-oxo-2H-chromen-3-ylcarbamoyl)acrylic acid methyl ester

References
References	Yi, L., et al. 2009. Angew Chem. Int. Ed. Engl. 48, 4034.

Product Information
CAS number	1173888-41-9
Form	Brown solid
Hill Formula	C₁₄H₁₁NO₆
Chemical formula	C₁₄H₁₁NO₆
Structure formula Image
Quality Level	MQ100

Applications

Biological Information
Purity	≥98% by HPLC

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	-20°C
Protect from Light	Protect from light
Do not freeze	Ok to freeze
Special Instructions	Use only fresh DMSO that for reconstitution. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Packaging Information
Packaged under inert gas	Packaged under inert gas

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
595504-10MGCN	04055977189094

Documentation

Thiol Fluorescent Probe IV - CAS 1173888-41-9 - Calbiochem MSDS

职位
物料安全数据表 (MSDS)

Thiol Fluorescent Probe IV - CAS 1173888-41-9 - Calbiochem 分析证书

标题	批号
595504	输入批号

参考

参考信息概述
Yi, L., et al. 2009. Angew Chem. Int. Ed. Engl. 48, 4034.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	19-April-2011 RFH
Synonyms	3-(7-Hydroxy-2-oxo-2H-chromen-3-ylcarbamoyl)acrylic acid methyl ester
Description	A cell-permeable, practically non-fluorescent (Φ₀ = 0.001) coumarin cis-acrylate derivative that exhibits a 470-fold increase in fluorescence property (Φ = 0.47; Ex. 400 nm, Em. 465 nm) upon an 1:1 Michael adduct formation with thiol/sulfhydryl and acts as a fast-reacting (t_1/2 = 3 msec with 50 µM probe and 50 mM 2-ME at t = 0), highly sensitive (Detection limit ≤0.5 nM GSH with 100 nM probe), and thiol-specific fluorogenic probe, displaying high reactivity toward 2-ME, GSH, and Cysteine, but not non-thiol-containing GSSG or Cystine. A more sensitive alternative to Coomassie Blue in staining of BSA on SDS-PAGE gels and has been applied successfully in fluorescent labeling cellular thiols in intact HEK293 cells as well as in detecting glutathione reductase activity in cell-free enzymatic assays. ThioGlo^®1 (Cat. No. 595501), ThioGlo^®3, and ThioGlo^®5, in comparison, exhibit much lower fluorescence quantum yield increases upon reaction with thiol (61-, 156-, and 28-fold increase, respectively).
Form	Brown solid
Intert gas (Yes/No)	Packaged under inert gas
CAS number	1173888-41-9
Chemical formula	C₁₄H₁₁NO₆
Structure formula
Purity	≥98% by HPLC
Solubility	DMSO (50 mg/ml)
Storage	Protect from light -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Use only fresh DMSO that for reconstitution. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity	Standard Handling
References	Yi, L., et al. 2009. Angew Chem. Int. Ed. Engl. 48, 4034.

种类

Life Science Research > Inhibitors and Biochemicals > Biochemicals > Dyes, Stains and Probes > Fluorescent Viability Stains and Intracellular Probes

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