Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Recombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. Referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune systems response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation.
Catalogue Number
654245
Brand Family
Calbiochem®
Synonyms
rmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α
References
References
Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293. Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.
Product Information
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.
ED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Purity
≥97% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤100 pg/µg TNF-α
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
≤ -70°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293. Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
19-May-2008 RFH
Synonyms
rmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α
Description
Recombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. It is referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune system's response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation.
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.
Purity
≥97% by SDS-PAGE
Contaminants
Endotoxin: ≤100 pg/µg TNF-α
Biological activity
ED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Solubility
Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Storage
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Toxicity
Standard Handling
Merck USA index
14, 9812
References
Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293. Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.
