Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Recombinant, human tumor necrosis factor-α expressed in E. coli. A cytokine produced primarily by activated macrophages that activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein kinase (SAPK).
Catalogue Number
654205
Brand Family
Calbiochem®
Synonyms
rhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
References
References
Merck Index 13, 9882. Tsuchida, H., et al. 1995. J. Immunol. 154, 2403. Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689. Polunovsky, V.A., et al. 1994. Exp. Cell Res.214, 584. Koide, M., et al. 1993. FEBS Lett.318, 213. Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem.266, 7313.
ED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Purity
≥95% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤0.1 ng/µg TNF-α
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
MT6435000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
≤ -70°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
Merck Index 13, 9882. Tsuchida, H., et al. 1995. J. Immunol. 154, 2403. Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689. Polunovsky, V.A., et al. 1994. Exp. Cell Res.214, 584. Koide, M., et al. 1993. FEBS Lett.318, 213. Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem.266, 7313.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
05-January-2009 JSW
Synonyms
rhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
Description
Recombinant, human tumor necrosis factor-α expressed in E. coli. Activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein (SAP) kinase.
Form
Lyophilized
Formulation
Carrier-free
CAS number
94948-59-1
RTECS
MT6435000
Purity
≥95% by SDS-PAGE
Contaminants
Endotoxin: ≤0.1 ng/µg TNF-α
Biological activity
ED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Solubility
Reconstitute with sterile, dH₂O to a final concentration of 0.1-1 mg/ml. Further dilutions should be made in low endotoxin medium or aqueous buffer containing carrier protein such as heat inactivated FCS or tissue culture grade BSA.
Storage
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
Toxicity
Standard Handling
References
Merck Index 13, 9882. Tsuchida, H., et al. 1995. J. Immunol. 154, 2403. Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689. Polunovsky, V.A., et al. 1994. Exp. Cell Res.214, 584. Koide, M., et al. 1993. FEBS Lett.318, 213. Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem.266, 7313.
