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14-749M TAO1 Protein, active, 250 µg

Active, N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327. For use in Kinase Assays.

TAO1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-749M
250 µg  Also available in 10 μg size (cat#14-749) and in bulk (cat# 14-749-K).
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      概述

      Replacement Information
      Description
      Catalogue Number14-749M
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionTAO1 Protein, active, 250 µg
      OverviewN-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327.
      References
      Product Information
      Presentation10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      PurityPurity 93% by SDS PAGE and Coomassie blue staining
      SourceExpressed by baculovirus in Sf21 cells.
      Specific Activity1179 U/mg, where one unit of TAO1 activity is defined as 1 nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.
      Entrez Gene Number
      Gene Symbol
      • TAOK1
      • MAP3K16
      • KIAA1361
      • PSK2
      • MARKK
      • FLJ14314
      • hKFC-B
      Protein TargetTAO1
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilySTE
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q7L7X3 # Phosphorylates MKK3 (By similarity). Activates the p38 MAP kinase pathway through the specific activation of the upstream MKK3 kinase.
      SIZE: 1001 amino acids; 116070 Da
      SUBUNIT: Self-associates. Interacts with MKK3 (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Highly expressed in the testis, and to a lower extent also expressed in brain, placenta, colon and skeletal muscle.
      PTM: Autophosphorylated (By similarity).
      SIMILARITY: SwissProt: Q7L7X3 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 protein kinase domain.
      Molecular Weight41 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of TAO1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
      Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-749) and in bulk (cat# 14-749-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      14-749M 04053252582455

      相关产品和应用

      Alternative Packsize

      产品目录编号 描述  
      14-749 TAO1 Protein, active, 10 µg 显示价格及库存情况

      产品族

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      种类

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases