Stericup® 过滤装置
Stericup真空抽滤系统包含一个Steritop真空抽装置和一个接收瓶,用来处理和储存150 mL至1,000 mL液体。
Stericup真空抽滤系统包含一个Steritop过滤装置和一个接收瓶,用来处理和储存150 mL至1,000 mL液体。
接收瓶设计新颖,不会倾倒,易于抓握,外形紧凑,在过滤过程中保持稳定,因此Stericup过滤装置特别适合在层流罩中使用。
另一个方便的特点是,接收瓶的底部稍微凹陷,盖上盖的瓶子可以方便地叠放储存。
Stericup真空抽滤系统包含一个Steritop过滤装置和一个接收瓶,用来处理和储存150 mL至1,000 mL液体。
接收瓶设计新颖,不会倾倒,易于抓握,外形紧凑,在过滤过程中保持稳定,因此Stericup过滤装置特别适合在层流罩中使用。
另一个方便的特点是,接收瓶的底部稍微凹陷,盖上盖的瓶子可以方便地叠放储存。 Less<<
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问与答
问题 | 回答 |
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When I use my Stericup/top, sometimes some of my sterilized solution gets pulled into my vacuum line. How can I eliminate this from happening? | Attach your vacuum tubing and turn the vacuum on before adding the liquid to the Stericup/top. This eliminates any initial solution that flowed through the membrane before starting the vacuum from being pulled into the vacuum line. |
What is the typical holdup volume for Stericup. | The hold up volume is about 6 ml. If you give the unit a slight shake, you can reduce the holdup volume by about 60% to 2.4 ml. Solutions containing protein have a slightly higher holdup volume, about 7 ml, again reduced to less than half by a slight shake. |
What is the diameter of the membrane in the Stericup and Steritop units? | All of the cups and tops currently have a 70mm diameter membrane, which has 43 cm2 of surface area. |
Why use a vacuum driven filter rather than a pressure driven filter? | Unlike positive pressure filtration, vacuum driven filtration requires only a vacuum source. It does not require pressure vessels or peristaltic tubing pumps. Stericup and Steritop units are pre-sterilized and disposable making setup and cleanup easy! |
Should I filter my acrylamide solution prior to gel casting? | Yes, filtering your acrylamide solution before adding the polymerization activator ammonium persulfate and TEMED will accomplish two things. 1. It will eliminate particulate matter that may interfere with base calling when sequencing or that may create "hot" spots in the gel, and 2. It will aid in degassing, which will promote efficient polymerization of the gel. Products appropriate for this application would be Stericup, Steritop or Steriflip with express membrane. |
It sounds like air is leaking into my Stericup, what should I do? | First make sure that the top is securely fastened to the base. Discontinue use if there appears to be any cracks on the apparatus. |
What Stericup would you recommend to remove mycoplasma? | The Stericup VP ontains an 0.1um Express membrane. The VP membrane has been validated for the removal of mycoplasma. |
Which pre-filter should I be using with my Stericup or Steritop unit? | You should use an AP20 75mm pre-filter (AP2007500). To help keep the filter from floating in the solution to be filtered, insert the pre-filter under the pre-filter tab inside the Stericup or Steritop. |
What is the expiration date on Stericup and Steritop units? | The expiration date, which specifies product sterility, for the Stericup and Steritop products is three years from the date of manufacture. |
What type of flow rates can I expect with the Stericups and Steritops? | For typical non -serum tissue culture media you can expect flow rates from 1-1.5 lpm. |