Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
The S•Tag™ Thrombin Purification Kit is designed for rapid affinity purification of S•Tag fusion proteins that also contain a sequence for thrombin cleavage. The S-protein Agarose specifically retains S•Tag fusion proteins. When fusion proteins are expressed from vectors that also encode a thrombin cleavage site (LVPR/GS) between the standard 15-amino acid S•Tag sequence (KETAAAKFERQHMDS) and the cloning region, thrombin digestion can release the target protein from the S-protein Agarose. This kit uses biotinylated thrombin to enable simple and specific thrombin removal with Streptavidin-Agarose, after the digestion step.
Manu Agarwal, et al. (2006) A R2R3-type myb transcription factor is involved in the cold-regulation of CBF genes and in acquired freezing tolerance. Journal of Biological Chemistry281, 37636-37645.
Kunhong Xiao, et al. (2004) Activation-dependent conformational changes in β-arrestin 2. Journal of Biological Chemistry279, 55744-55753.
