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Recombinant, mouse sclerostin (SOST) consisting of amino acids 24-211, expressed in NS0-derived murine myeloma cells with an N-terminal His•Tag®. SOST is expressed by osteoclasts in developing bones of mouse embryos and acts as a negative regulator of bone formation through repression of BMP-induced osteogenesis.
Catalogue Number
567693
Brand Family
Calbiochem®
References
References
Kusu, N. et al. 2003. J. Biol. Chem.278, 24113. Balemans, W. et al. 2001. Hum. Mol. Genet.10 537. Brunkow, M.E. et al. 2001. Am. J. Hum. Genet. 68, 577.
Product Information
Form
Solid
Formulation
Lyophilized from 0.2 µm filter-sterilized PBS, BSA.
Inhibition Assay: Inhibits hBMP-6-induced alkaline phosphatase by 50-80% in MC3T3-E1 mouse osteoblastic cells when used at a concentration of 30 µg/ml.
Purity
≥90% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Kusu, N. et al. 2003. J. Biol. Chem.278, 24113. Balemans, W. et al. 2001. Hum. Mol. Genet.10 537. Brunkow, M.E. et al. 2001. Am. J. Hum. Genet. 68, 577.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
21-Sepmtember-2011 RFH
Description
Recombinant, mouse sclerostin (SOST) consisting of amino acids 24-211, expressed in NS0-derived murine myeloma cells with an N-terminal His•Tag®. SOST is expressed by osteoclasts in developing bones of mouse embryos and acts as a negative regulator of bone formation through repression of BMP-induced osteogenesis.
Form
Solid
Formulation
Lyophilized from 0.2 µm filter-sterilized PBS, BSA.
Purity
≥90% by SDS-PAGE
Contaminants
Endotoxin: <1.0 EU/µg protein
Biological activity
Inhibition Assay: Inhibits hBMP-6-induced alkaline phosphatase by 50-80% in MC3T3-E1 mouse osteoblastic cells when used at a concentration of 30 µg/ml.
Solubility
Reconstitute to 200 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Kusu, N. et al. 2003. J. Biol. Chem.278, 24113. Balemans, W. et al. 2001. Hum. Mol. Genet.10 537. Brunkow, M.E. et al. 2001. Am. J. Hum. Genet. 68, 577.
