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Native homodimeric S-100b isoform composed of ββ-subunits, purified from bovine brain. Elevated serum level of S-100b is used as a marker of stroke and cerebral edema.
Catalogue Number
559290
Brand Family
Calbiochem®
References
References
Momotani, E., et al. 1993. J. Comp. Pathol. 108, 291. Baudier, J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 11627. Donato, R. 1991. Cell Calcium12, 713. Isobe, T., et al. 1977. Biochim. Biophys. Acta494, 222.
Product Information
Form
Lyophilized
Formulation
Lyophilized from 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 100 µM DTT.
Momotani, E., et al. 1993. J. Comp. Pathol. 108, 291. Baudier, J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 11627. Donato, R. 1991. Cell Calcium12, 713. Isobe, T., et al. 1977. Biochim. Biophys. Acta494, 222.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
27-January-2014 JSW
Description
The homodimeric S-100 isoform composed of ββ-subunits, purified from bovine brain. The S-100 proteins are acidic Ca2+ binding proteins found predominately in glial cells. Evidence indicates that upon binding to Ca2+, S-100 proteins expose a hydrophobic domain to the solvent in a manner similar to calmodulin. Within the cell, S-100 proteins have been reported to regulate adenylate cyclase, aldolase activities, ATPase, microtubules, and protein phosphorylation. Under nonreducing conditions both the dimeric and monomeric forms may be visible.
Note: This product is not to be used for animal treatment, in vivo research or in any other contact procedure with livestock.
Form
Lyophilized
Formulation
Lyophilized from 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 100 µM DTT.
Purity
≥95% by SDS-PAGE
Contaminants
S100 α-chain: ≤10% by Western Blot
Solubility
50 mM Tris buffer, pH 8.0 (5 mg/ml)
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity
Standard Handling
References
Momotani, E., et al. 1993. J. Comp. Pathol. 108, 291. Baudier, J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 11627. Donato, R. 1991. Cell Calcium12, 713. Isobe, T., et al. 1977. Biochim. Biophys. Acta494, 222.
