Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
An ultrasensitive Coomassie-based reagent for staining polyacrylamide gels. No mixing or preparation is involved, no fixation step is needed, and no destaining is required. RAPIDstain™ Reagent stains only protein, within 5-10 min, and leaves a crystal-clear background with high resolution. Note: 1 liter is sufficient to stain up to 20 minigels or 10 standard (16 x 18 cm) gels.
Harmful by inhalation and if swallowed. Irritating to eyes, respiratory system and skin.
S Phrase
S: 36-45
Wear suitable protective clothing. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Harmful
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
17-September-2007 RFH
Description
An ultrasensitive Coomassie-based reagent for staining polyacrylamide gels. No mixing or preparation is involved, no fixation step is needed, and no destaining is required. RAPIDstain™ Reagent stains only protein, within 5-10 min, and leaves a crystal-clear background with high resolution. Contain the following component:
• RAPIDstain™ 1 x 1 liter Suitable for 20 mini gels (8 x 10 cm) or 10 larger gels (12 x 15 cm)
Form
Liquid
Recommended reaction conditions
1. Wash gel three times in ultra-pure water, 5-10 min per wash.
NOTE:
• Use 100 ml for each 8 x 10 cm mini-gel.
• Isoelectric focusing gels require pre-fixing in 20% TCA for 30 min, followed by extensive washing.
2. Discard all free water from the gel. Add RAPIDStain™ to cover the gel. Gently shake gel in stain for about an h.
NOTE:
• Protein bands will be visible within 10 min and reach maximum intensity within 1 h. Longer incubation periods will not increase the background.
3. Wash gel two to three times in deionized water. Washing enhances the staining of weak protein bands. Store gel in water.
NOTE:
• If background staining is noticed then it is indicative of residual SDS in gel. Rinsing the gel extensively in ultra-pure water will remove the background staining.
