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Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low density lipoprotein receptor-related protein (LRP) and very low-density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low density lipoprotein receptor (LDLR) and several of its class 2 mutants. Expressed as a GST-fusion protein that is subsequently cleaved with thrombin. Typical concentrations to block LRP or VLDL receptor are 200-300 nM. For antagonism of LDLR, 1 µM is recommended.
Catalogue Number
553506
Brand Family
Calbiochem®
Synonyms
Receptor Associated Protein
References
References
Li, Y., et al. 2002. Biochemistry41, 4921. Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160. Anderson, O.M., et al. 2001. Biochemistry40, 15408. Bu, G. 2001. Int. Rev. Cytol.209, 79. Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035
Li, Y., et al. 2002. Biochemistry41, 4921. Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160. Anderson, O.M., et al. 2001. Biochemistry40, 15408. Bu, G. 2001. Int. Rev. Cytol.209, 79. Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
20-April-2009 JSW
Synonyms
Receptor Associated Protein
Description
Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Receptor Associated Protein (RAP) antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low-density lipoprotein receptor-related protein (LRP) and very low density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low-density lipoprotein receptor (LDLR) and several of its class 2 mutants. Typical concentrations used to block LRP or VLDL receptor are 200-300 nM; for antagonism of LDLR, 1 µM is recommended.
Form
Liquid
Formulation
150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
Concentration Label
Please refer to vial label for lot-specific concentration
Purity
≥90% by SDS-PAGE
Storage
Avoid freeze/thaw
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Li, Y., et al. 2002. Biochemistry41, 4921. Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160. Anderson, O.M., et al. 2001. Biochemistry40, 15408. Bu, G. 2001. Int. Rev. Cytol.209, 79. Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035
