Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Contains five highly purified proteins ranging in molecular weight from 12.4 kDa to 290 kDa. Formulated to give peaks of approximately the same intensity for each protein when run on a TSK-Gel G300SW column after reconstituting with elution buffer and monitored at 280 nm. Markers include: cytochrome c, 12,400; myokinase, 32,000; enolase, 67,000; lactate dehydrogenase, 142,000; and glutamate dehydrogenase, 290,000.
Note: 5T = 5 Tests.
Catalogue Number
539053
Brand Family
Calbiochem®
Application Data
References
Product Information
Form
Lyophilized
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 weeks at -20°C.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
30-March-2009 JSW
Application Data
Description
Contains five highly purified proteins ranging in molecular weight from 12.4 kDa to 290 kDa. Formulated to give peaks of approximately the same intensity for each protein when run on a TSK-Gel G300SW* column after reconstituting with elution buffer and monitored at 280 nm.
Each vial contains sufficient material for approximately 5–10 tests. There is 200 µg of each protein per vial x 5 proteins = 1000 µg total protein per vial. Each vial also contains ~5 mg sucrose as a carrier.
*Toyosoda Industry Co., Ltd.
Form
Lyophilized
Solubility
Reconstitute with 100 µl of 100 mM sodium phosphate buffer, pH 7.0 containing 200 mM NaCl. If the material is reconstituted with lower ionic strength buffer (i.e., <0.1 M NaCl) the cytochrome c may interact nonspecifically with the silica gel column and migrate incorrectly. Apply 20 µl to an analytical grade column such as TSK-Gel G3000SW (0.75 x 60 cm).
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 weeks at -20°C.
