Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Binds ≥99% to human IgG immobilized on a column. o-Phenylenediamine, 1 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C, is used for testing immunoenzyme reactivity Purity of the recombinant protein G has been determined by presence of a single band at M.W. 33,800 on a 15% SDS-polyacrylamide gel. Specifically binds the Fc region of IgG from various species. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Purity
Single band at 33.8 kDa by SDS-PAGE
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Yes
Mercury prohibited
To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
01-March-2021 JSW
Application
Direct Immunoassay (1:5000)
Description
Purified recombinant protein G conjugated to peroxidase.
Background
Protein G is a cell-surface protein of Streptococcus species that binds to the Fc portions of immunoglobulins from various species. Like protein A, it is a versatile and economic means to isolate, detect and purify antibodies produced by many animals. Protein G is especially valued because of its ability to react with Human IgG3 and a number of animal immunoglobulins with which protein A does not react well (i.e. bovine, goat, horse, rat and sheep). It also reacts more strongly with mouse immunoglobulins than protein A. Additionally, unlike protein A, it does not react with IgA, IgE and IgM.
Form
Liquid
Formulation
In 0.01 M PBS, pH 7.6, 10% mannose, 2% sucrose, 0.5 mM EDTA, 1% BSA, 0.1% Kathon
Concentration Label
Please refer to vial label for lot-specific concentration
Purity
Single band at 33.8 kDa by SDS-PAGE
Comments
Binds ≥99% to human IgG immobilized on a column. o-Phenylenediamine, 1 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C, is used for testing immunoenzyme reactivity Purity of the recombinant protein G has been determined by presence of a single band at M.W. 33,800 on a 15% SDS-polyacrylamide gel. Specifically binds the Fc region of IgG from various species. Variables associated with assay conditions will dictate the proper working dilution.
