Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
A kit suitable for quantitative array of a wide variety of proteases in biological samples, utilizing FTC-casein as a substrate. Enzyme activities that have been detected with this assay kit include: chymotrypsin, elastase, plasminogen, PRONASE® Protease, subtilisin, thermolysin, and trypsin.
Catalogue Number
539125
Brand Family
Calbiochem®
Materials Required but Not Delivered
• Trichloroacetic acid (TCA), 5% • Spectrophotometer that can accurately measure the absorbance at 492 nm • Pipetting devices for accurate delivery of volumes required for the assay • Cuvettes with optical properties suitable for use at 492 nm • A constant-temperature water bath set at 37°C • Microfuge for generating approximately 12,000 x g
References
References
Twining, S.S. 1984. Anal. Biochem.143, 30. Wiesner, R., et al. 1982. Anal. Biochem.121, 290.
Product Information
Detection method
Colorimetric
Form
200 Tests
Format
Cuvette or 96-well plate
Kit contains
A Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
• FTC-casein should be stored at -20°C and is stable for one year from the date of purchase. FTC-casein stored at 4°C is stable for 2-3 days. Several freeze-thaw cycles will lead to a minor increase in the background fluorescence, so following initial thawing, we suggest that this reagent be divided into 5 aliquots. FTC-casein is light sensitive and should be protected from light. • Protease positive control should be stored at -20°C. As with many proteases, trypsin is susceptible to autolysis and you may notice a reduction in trypsin activity with time, even if stored at -20°C. • Incubation buffer and assay buffer may be stored at 4°C or -20°C.
Protect from Light
Protect from light
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains
A Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
