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Native Glu-type plasminogen from human plasma. Plasminogen is a single chain glycoprotein found in normal plasma at ~12 mg per deciliter, and is the inactive precursor of the serine protease plasmin.
Catalogue Number
528180
Brand Family
Calbiochem®
References
References
Collen, D. 1986. In Blood Coagulation (Zwaal, R.E.A., and Hemker, H.C. eds.) Elsevier, N.Y. p. 243. Barlow, G.H., et al. 1984. Biochemistry23, 2384. Wohl, R.C., et al. 1982. Thromb. Res.27, 523. Castellino, F.J., et al. 1981. Methods Enzymol.80, 365. Robbins, K.C. 1976. Methods Enzymol.45, 257.
Collen, D. 1986. In Blood Coagulation (Zwaal, R.E.A., and Hemker, H.C. eds.) Elsevier, N.Y. p. 243. Barlow, G.H., et al. 1984. Biochemistry23, 2384. Wohl, R.C., et al. 1982. Thromb. Res.27, 523. Castellino, F.J., et al. 1981. Methods Enzymol.80, 365. Robbins, K.C. 1976. Methods Enzymol.45, 257.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
16-May-2008 RFH
Description
Native Glu-type plasminogen from human plasma. Plasminogen is a single chain glycoprotein zymogen, which is synthesized in the liver and circulates in plasma at a concentration of approximately 2.4 µM. The plasminogen molecule contains 790 amino acids, 24 disulfide bridges, no free sulfhydryls, and 5 regions of internal sequence homology, known as kringles, between Lys77 and Arg560. These kringle regions are homologous to the kringle domains in t-PA, u-PA, and prothrombin. Native Glu-plasminogen is readily converted to Lys77-plasminogen by plasmin hydrolysis of the Lys76-Lys77 peptide bond. The plasminogen is converted to plasmin by cleavage at Arg540-Val561 by urokinase, streptokinase, or tissue plasminogen activator.
Form
Liquid
Formulation
In 100 mM NaCl, 50 mM Tris-HCl, pH 7.5.
Source
Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Purity
Single band by SDS-PAGE
Contaminants
Plasmin activity: none detected
Storage
Avoid freeze/thaw
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Irritant
Merck USA index
14, 7521
References
Collen, D. 1986. In Blood Coagulation (Zwaal, R.E.A., and Hemker, H.C. eds.) Elsevier, N.Y. p. 243. Barlow, G.H., et al. 1984. Biochemistry23, 2384. Wohl, R.C., et al. 1982. Thromb. Res.27, 523. Castellino, F.J., et al. 1981. Methods Enzymol.80, 365. Robbins, K.C. 1976. Methods Enzymol.45, 257.
