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Stimulates the proliferation of human lymphocytes and mouse splenic T cells. Causes agglutination titers of 1:128 in tests using 2% solution of type A red blood cells.
Catalogue Number
526511
Brand Family
Calbiochem®
References
References
Dupis, G., and Leclair, B. 1982. FEBS Lett. 144, 29. Felsted, R.L., et al. 1977. J. Biol. Chem. 252, 2967.
Dupis, G., and Leclair, B. 1982. FEBS Lett. 144, 29. Felsted, R.L., et al. 1977. J. Biol. Chem. 252, 2967.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
07-May-2018 JSW
Description
T-cell mitogen that stimulates the proliferation of human lymphocytes and mouse splenic T-cells. PHA is a mixture of tetramers consisting of combinations of two subunits, E and L, with different biological activity. E-4 isolectin has low mitogenic activity and high erythroagglutinating activity. L-4 isolectin has high mitogenic and leukoagglutinating activities and low erythroagglutinating activity. Causes agglutination titers of 1:128 in tests that use a 2% solution of type A red blood cells. Inhibited by N-acetyl-D-galactosamine, peptides containing the sequence NANA-D-Gal-(1 → 3,4), GlcNAcα(1 → 2), or Man α(1 → 2)Man.
Form
Lyophilized
CAS number
9008-97-3
RTECS
TJ3470000
Biological activity
Optimum activity for the stimulation of proliferation of lymphocytes is
at 5-10 µg/ml.
Solubility
Sterile redistilled H₂O (2-10 mg/ml)
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Unstable in solution; reconstitute just prior to use.
Toxicity
Standard Handling
Merck USA index
14, 5429
References
Dupis, G., and Leclair, B. 1982. FEBS Lett. 144, 29. Felsted, R.L., et al. 1977. J. Biol. Chem. 252, 2967.