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Native phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline. Coupled with choline oxidase, it can also be used for the determination of phospholipids in serum.
Catalogue Number
525200
Brand Family
Calbiochem®
Synonyms
PLD
References
References
Kurz, T., et al. 1993. Circ. Res. 72, 701. Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337. Yamamoto, I., et al. 1993. Biochim. Biophys. Acta1145, 293.
Product Information
CAS number
9001-87-0
Activity
≥50 units/mg dry weight
Unit of Definition
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphatidylcholine per min at 37°C, pH 8.0.
Harmful by inhalation, in contact with skin and if swallowed.
S Phrase
S: 36
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Harmful
Storage
-20°C
Protect from Moisture
Protect from moisture
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 6 months at 4°C or for up to 1 year at -20°C.
Kurz, T., et al. 1993. Circ. Res. 72, 701. Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337. Yamamoto, I., et al. 1993. Biochim. Biophys. Acta1145, 293.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
26-August-2008 RFH
Synonyms
PLD
Description
Native phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline; coupled with choline oxidase, it can be used for the determination of phospholipids in serum.
Form
Lyophilized brownish solid
CAS number
9001-87-0
EC number
3.1.4.4
Contaminants
Aatalase, glucose oxidase: none detected
Activity
≥50 units/mg dry weight
Unit definition
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphatidylcholine per min at 37°C, pH 8.0.
Solubility
10 mM Tris-HCl, pH 8.0, 0.05% BSA, and 0.1% (w/v) TRITON® X-100 (1 mg/ml)
Storage
Protect from moisture
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 6 months at 4°C or for up to 1 year at -20°C.
Toxicity
Harmful
References
Kurz, T., et al. 1993. Circ. Res. 72, 701. Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337. Yamamoto, I., et al. 1993. Biochim. Biophys. Acta1145, 293.
