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Full-length, recombinant, human phosphodiesterdase 7A1 (PDE7A1) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE7A1 is required for T-cell activation induced by the costimulation of CD3 and CD28 receptors. Increased PDE7A1 correlates with a decrease in cAMP, increased IL-2, and increased cell proliferation. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.
Catalogue Number
524751
Brand Family
Calbiochem®
Synonyms
hPDE7A1
Application Data
Increasing concentrations of SB36207, a PDE7A1 inhibitor, were mixed with the enzyme and the activity was measured.
References
References
Han, P., et al. 2006. J. Biol. Chem.281, 15050. Lugnier, C. 2006. Pharmacol Ther.109, 366. Bloom, T.J., and Beavo, J.A. 1996. Proc. Natl. Acad. Sci. U S A93, 14188.
Product Information
Activity
≥ 0.5 U/µl
Unit of Definition
One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.
Phosphodiesterase 7A1, Human, Recombinant, S. frugiperda 分析证书
标题
批号
524751
参考
参考信息概述
Han, P., et al. 2006. J. Biol. Chem.281, 15050. Lugnier, C. 2006. Pharmacol Ther.109, 366. Bloom, T.J., and Beavo, J.A. 1996. Proc. Natl. Acad. Sci. U S A93, 14188.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
17-December-2008 JSW
Synonyms
hPDE7A1
Application Data
Increasing concentrations of SB36207, a PDE7A1 inhibitor, were mixed with the enzyme and the activity was measured.
Description
Full-length, recombinant, human phosphodiesterdase 7A1 (PDE7A1) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE7A1 is required for T-cell activation induced by the costimulation of CD3 and CD28 receptors. Increased PDE7A1 correlates with a decrease in cAMP, increased IL-2, and increased cell proliferation. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.
Form
Liquid
Formulation
In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.
Activity
≥ 0.5 U/µl
Unit definition
One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.
Storage
Avoid freeze/thaw
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Han, P., et al. 2006. J. Biol. Chem.281, 15050. Lugnier, C. 2006. Pharmacol Ther.109, 366. Bloom, T.J., and Beavo, J.A. 1996. Proc. Natl. Acad. Sci. U S A93, 14188.
