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Full-length, recombinant, human phosphodiesterdase 3A (PDE3A) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE3A is expressed primarily in cardiac and vascular myocytes and platelets. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.
Catalogue Number
524742
Brand Family
Calbiochem®
Synonyms
cAMP-specific phosphodiesterase 3A, PDE3A
Application Data
Increasing concentrations of the PDE3 inhibitor, cilostazol, were added to PDE3A and the activity measured.
References
References
Shakur, Y., et al. 2000. J. Biol. Chem.275, 38749.
Product Information
Activity
≥ 0.5 U/µl
Unit of Definition
One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at pH 7.5, 30°C.
Phosphodiesterase 3A, Active, Human, Recombinant, S. frugiperda 分析证书
标题
批号
524742
参考
参考信息概述
Shakur, Y., et al. 2000. J. Biol. Chem.275, 38749.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
03-February-2009 JSW
Synonyms
cAMP-specific phosphodiesterase 3A, PDE3A
Application Data
Increasing concentrations of the PDE3 inhibitor, cilostazol, were added to PDE3A and the activity measured.
Description
Full-length, recombinant, human phosphodiesterdase 3A (PDE3A) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE3A is expressed primarily in cardiac and vascular myocytes and platelets. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.
Formulation
In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.
Activity
≥ 0.5 U/µl
Unit definition
One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at pH 7.5, 30°C.
Storage
Avoid freeze/thaw
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Shakur, Y., et al. 2000. J. Biol. Chem.275, 38749.