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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

506119 Sigma-AldrichPhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb

This PhosphoDetect™ Anti-p38 MAP Kinase Rabbit pAb is validated for use in WB, ICC for the detection of p38 MAP Kinase .

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
Ca, H, M, R	Rb	Polyclonal Antibody

价格及供货情况

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Description
Overview	Recognizes p38α, β, and γ/ERK6 phosphorylated at Thr¹⁸⁰ and Tyr¹⁸² in uv-treated HEK293 cells and sorbitol-treated PC12 cells.
Overview	Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Catalogue Number	506119
Brand Family	Calbiochem®
Application Data	Detection of human p38 MAP kinase phosphorylated at Thr¹⁸⁰/Tyr¹⁸², by immunoblotting. Sample: Whole cell lysate from HEK293 cells treated with UV irradiation. Primary antibody: PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb (Cat. No. 506119) (1:1000) pre-incubated with peptide immunogen (lane 1), a generic phophothreonine-containing peptide (lane 2), a generic phosphotyrosine-containg peptide (lane 3), non-phosphorylated peptide corresponding to the phosphopeptide (lane 4), without peptide (lane 5), with phophopeptide derived from the corresponding region of JNK1&2 (lane 6), and phosphopetide derived from the corresponding region of ERK1&2 (lane 7). Detection: chemiluminescence.

References
References	Marinissen, M.J., et al. 2001. Genes Dev. 15, 535. Nakagami, H., et al. 2001. Diabetes 50, 1472. Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988. Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420. Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531. Han, J., et al. 1994. Science 265, 808. Lee, J.C., et al. 1994. Nature 372, 739. Rouse, J., et al. 1994. Cell 78, 1027.

References

Marinissen, M.J., et al. 2001. Genes Dev. 15, 535.
Nakagami, H., et al. 2001. Diabetes 50, 1472.
Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988.
Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
Han, J., et al. 1994. Science 265, 808.
Lee, J.C., et al. 1994. Nature 372, 739.
Rouse, J., et al. 1994. Cell 78, 1027.

Product Information
Form	Liquid
Formulation	In Dulbecco's PBS (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Positive control	UV irradiated HEK293 cells or sorbitol-treated PC12 cells
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunocytochemistry
Application Notes	Immunoblotting (1:1000) Immunocytochemistry (see comments)
Application Comments	Does not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
Immunogen	Human
Host	Rabbit
Isotype	IgG
Species Reactivity	Canine Human Mouse Rat
Antibody Type	Polyclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
506119-10TCN	04055977199451

Documentation

PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb MSDS

职位
物料安全数据表 (MSDS)

PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb 分析证书

标题	批号
506119	输入批号

参考

参考信息概述
Marinissen, M.J., et al. 2001. Genes Dev. 15, 535. Nakagami, H., et al. 2001. Diabetes 50, 1472. Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988. Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420. Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531. Han, J., et al. 1994. Science 265, 808. Lee, J.C., et al. 1994. Nature 372, 739. Rouse, J., et al. 1994. Cell 78, 1027.

参考信息概述

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	08-August-2007 RFH
Application	Immunoblotting (1:1000) Immunocytochemistry (see comments)
Application Data	Detection of human p38 MAP kinase phosphorylated at Thr¹⁸⁰/Tyr¹⁸², by immunoblotting. Sample: Whole cell lysate from HEK293 cells treated with UV irradiation. Primary antibody: PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb (Cat. No. 506119) (1:1000) pre-incubated with peptide immunogen (lane 1), a generic phophothreonine-containing peptide (lane 2), a generic phosphotyrosine-containg peptide (lane 3), non-phosphorylated peptide corresponding to the phosphopeptide (lane 4), without peptide (lane 5), with phophopeptide derived from the corresponding region of JNK1&2 (lane 6), and phosphopetide derived from the corresponding region of ERK1&2 (lane 7). Detection: chemiluminescence.
Description	Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~p38 MAPK phosphorylated at Thr¹⁸⁰ and Tyr¹⁸².
Background	p38 MAP kinase (MAPK), also called RK and CSBP, is the mammalian homologue of yeast HOG kinase and participates in a cascade that controls cellular responses to cytokines and stress. Like the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light, and growth factors. Both MKK3 and SEK phosphorylate p38 on tyrosine and threonine resulting in p38 activation. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase-2 and to phosphorylate the transcription factors ATF-2 and Max.
Host	Rabbit
Immunogen species	Human
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
Isotype	IgG
Species	canine, human, mouse, rat
Positive control	UV irradiated HEK293 cells or sorbitol-treated PC12 cells
Form	Liquid
Formulation	In Dulbecco's PBS (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Preservative	≤0.1% sodium azide
Comments	Does not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Marinissen, M.J., et al. 2001. Genes Dev. 15, 535. Nakagami, H., et al. 2001. Diabetes 50, 1472. Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988. Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420. Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531. Han, J., et al. 1994. Science 265, 808. Lee, J.C., et al. 1994. Nature 372, 739. Rouse, J., et al. 1994. Cell 78, 1027.

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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