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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

676604 Sigma-AldrichPhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)

This PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Anti-Vasodilator-stimulated Phosphoprotein, Activated

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H, M	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.
Catalogue Number	676604
Brand Family	Calbiochem®
Synonyms	Anti-Vasodilator-stimulated Phosphoprotein, Activated
Application Data	Detection of human VASP, phosphorylated on Ser¹⁵⁷, by immunoblotting. Samples: Whole cell extracts from human platelets cells left untreated (lane 1) or SNP treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) (Cat. No. 676604) (0.5 µg/ml). Detection: chemiluminescence.

References
References	Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol. 13, 158. Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol. 166, 5550. Burkhardt, M., et al. 2000. J. Biol. Chem. 275, 33536. Harbeck, B., et al. 2000. J. Biol. Chem. 275, 40. Smolenski, A., et al. 2000. J. Biol. Chem. 275, 25723.

Product Information
Form	Lyophilized
Formulation	Lyophilized from PBS, PEG, sucrose.
Positive control	Vanadate-treated A431 cells
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay Immunoblotting (Western Blotting) Immunocytochemistry Immunoprecipitation
Application Notes	ELISA (0.05 µg/ml) Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence) Immunocytochemistry (1-10 µg/ml) Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Application Comments	Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
Immunogen	Human
Clone	5C6
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	-20°C
Do not freeze	Ok to freeze
Special Instructions	Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
676604-100UGCN	04055977260564

Documentation

PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) MSDS

职位
物料安全数据表 (MSDS)

PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) 分析证书

标题	批号
676604	输入批号

参考

参考信息概述
Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol. 13, 158. Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol. 166, 5550. Burkhardt, M., et al. 2000. J. Biol. Chem. 275, 33536. Harbeck, B., et al. 2000. J. Biol. Chem. 275, 40. Smolenski, A., et al. 2000. J. Biol. Chem. 275, 25723.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	15-September-2009 JSW
Synonyms	Anti-Vasodilator-stimulated Phosphoprotein, Activated
Application	ELISA (0.05 µg/ml) Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence) Immunocytochemistry (1-10 µg/ml) Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Application Data	Detection of human VASP, phosphorylated on Ser¹⁵⁷, by immunoblotting. Samples: Whole cell extracts from human platelets cells left untreated (lane 1) or SNP treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) (Cat. No. 676604) (0.5 µg/ml). Detection: chemiluminescence.
Description	Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.
Background	VASP (vasodilator-stimulated phosphoprotein), an established substrate of cAMP- and cGMP-dependent protein kinases in vitro and in living cells, is associated with focal adhesions, microfilaments, and membrane regions of high dynamic activity. VASP binds in vitro to profilin, zyxin, vinculin, and the Listeria sp. surface protein ActA. VASP also promotes actin nucleation and binds to actin filaments in vitro and associates with stress fibers in cells. Studies imply that VASP phosphorylation may act as an immediate negative regulator of actin dynamics. Phosphorylation of Ser¹⁵⁷ in VASP can be used as a measure of PKA activity.
Host	Mouse
Immunogen species	Human
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
Clone	5C6
Isotype	IgG₁
Species	human, mouse
Positive control	Vanadate-treated A431 cells
Form	Lyophilized
Formulation	Lyophilized from PBS, PEG, sucrose.
Preservative	≤0.1% sodium azide
Comments	Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
Storage	-20°C
Do Not Freeze	Ok to freeze
Special Instructions	Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Toxicity	Standard Handling
References	Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol. 13, 158. Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol. 166, 5550. Burkhardt, M., et al. 2000. J. Biol. Chem. 275, 33536. Harbeck, B., et al. 2000. J. Biol. Chem. 275, 40. Smolenski, A., et al. 2000. J. Biol. Chem. 275, 25723.

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种类

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