数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

525295 Sigma-AldrichPhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20)

This PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
A Broad Range Of Species	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Catalogue Number	525295
Brand Family	Calbiochem®

References
References	Glenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.

Product Information
Form	Liquid
Formulation	In 10 mM sodium phosphate, pH 7.4.
Positive control	Any cell lysate or tissue extract
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Immunoblotting Dixon, B.S., et al. 1996. J. Biol. Chem. 271, 13324. Frozen Sections, Immunocytochemistry Bao, Z.Z., et al. 1993. J. Cell Sci. 106, 579.
Key Applications	Enzyme-Linked Immunosorbent Assay Frozen Sections Immunoblotting (Western Blotting) Immunocytochemistry Immunoprecipitation
Application Notes	ELISA (see comments) Immunoblotting (1:500-1:1000, see application references) Frozen Sections (see application references) Immunocytochemistry (see application references) Immunoprecipitation (see comments)
Application Comments	Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	KLH conjugated to phosphotyrosine, glycine, and alanine
Clone	PY20
Host	Mouse
Isotype	IgG_2b
Species Reactivity	A Broad Range Of Species
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
525295-100UGCN	04055977270686

Documentation

PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) MSDS

职位
物料安全数据表 (MSDS)

PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) 分析证书

标题	批号
525295	输入批号

参考

参考信息概述
Glenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	03-December-2007 JSW
Application	ELISA (see comments) Immunoblotting (1:500-1:1000, see application references) Frozen Sections (see application references) Immunocytochemistry (see application references) Immunoprecipitation (see comments)
Description	Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
Background	Cellular activation, proliferation and differentiation are signaled by phosphorylation of tyrosine residues. Growth factor receptors and oncogene protein kinases are associated with tyrosine phosphorylation. Anti-phosphotyrosine antibodies are indispensable for the study of cellular tyrosine phosphorylation.
Host	Mouse
Immunogen	KLH conjugated to phosphotyrosine, glycine, and alanine
Clone	PY20
Isotype	IgG_2b
Species	a broad range of species
Positive control	Any cell lysate or tissue extract
Form	Liquid
Formulation	In 10 mM sodium phosphate, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Glenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
Application references	Immunoblotting Dixon, B.S., et al. 1996. J. Biol. Chem. 271, 13324. Frozen Sections, Immunocytochemistry Bao, Z.Z., et al. 1993. J. Cell Sci. 106, 579.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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